Cell storage and transportation medium, system, and method of cell aggregates

A method for transporting cells comprising transporting at least one cell storage and transportation system, the at least one cell storage and transportation system comprising:

• cells;
• a cell culture article including a chamber comprising an array of microcavities;
• and
• a cell storage and transport medium disposed in one or more of the microcavities and comprising a mixture of cell culture medium, agarose and methylcellulose, wherein a final agarose concentration in the cell storage and transport medium is about 0.5 to about 1.0% and a final methylcellulose concentration in the cell storage and transport medium is about 0.5 to about 0.7%;
• wherein the cells are suspended in the cell storage and transport medium;
• wherein each microcavity is structured to constrain the cells to grow in a three-dimensional spheroid conformation.