Adjustable loading device

An adjustable loading device comprising:

• a platform comprising at least two protrusions coupled thereto or positioned adjacent thereto, wherein at least one of the at least two protrusions is movable relative to the platform;
• and
• an energy source positioned adjacent to the platform and configured to direct energy toward the platform;
• and
• wherein the at least two protrusions are configured to receive tubing therebetween such that movement of the at least one movable protrusion adjusts a shape of the tubing.

A method of treating cells comprising:

• providing a mixture of cells mixed with microbubbles and at least one cyroprotective agent;
• introducing the mixture into a portion of tubing, where at least part of the portion of tubing is located in an adjustable loading device;
• providing the adjustable loading device comprising:
• a bottom platform comprising a plurality of rollers and at least one movable section;
• wherein the at least one movable section is configured to move from a first position to a second position;
• and
• an upper platform disposed above the bottom platform and comprising an energy emitting device that is positioned to emit energy toward the bottom platform;
• and
• causing the energy source to emit energy toward the portion of tubing, which is configured to cause the microbubbles in the mixture to oscillate and cause cavitation of cell membranes, such that the at least one cryoprotective agent is introduced into the cells.