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US 2026/0286271 A1Published application
Devices, systems and methods for aseptically sampling cell cultures
AssigneeKite Pharma, Inc.
InventorsOliver D. Lin, Dmitry Fradkin
Life of this patent
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Abstract
The present disclosure describes technology for sampling a fluid from a cell culture system or device. In various embodiments, the described methods may be performed by the described system in an automated or semi-automated fashion, thereby, reducing time and expense. In accordance with the described technology, samples may then be analyzed.
Claims23
A system for sampling a cell culture fluid, comprising:
- a fluidic assembly, comprising:
- a first fluid channel having a first end and a second end;
- a second fluid channel having a first end and a second end;
- a third fluid channel having a first end and a second end;
- and
- a sample chamber having a predefined volume, wherein the sample chamber is fluidically coupled to the second end of the first fluid channel at a first position, the second end of the second fluid channel at a second position, and the second end of the third fluid channel at a third position;
- wherein the fluidic assembly is mounted to a fluidic handling system, the fluidic handling system comprising:
- a first valve in physical communication between the first and second ends of the first fluid channel;
- and
- a second valve in physical communication between the first and second ends of the second fluid channel.
7 more elements
(canceled)
(canceled)
A method for sampling a cell culture fluid, comprising:
- applying a negative pressure to draw a cell culture sample from a cell culture vessel into a sample chamber through a first channel until the cell culture sample occupies a predefined volume of the sample chamber;
- actuating a first valve to a closed position to prevent a backflow of the cell culture sample from the sample chamber into the cell culture vessel;
- actuating a second valve to an open position to allow access of the cell culture sample from the sample chamber to a collection device through a second channel;
- and
- applying a positive pressure to push the cell culture sample from the sample chamber to the collection device through the second channel.
2 more elements
23-44. (canceled)
Dependencies are inferred from the claim text.