Methods for performing perfusion cell culture
AssigneeMerck Patent GmbH
InventorsJochen Bastian SIECK, Mona BAUSCH
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Abstract
The present invention relates to methods and media for performing perfusion cell culture whereby a concentrated medium supplement comprising phosphotyrosine is added to the perfusion cell culture in addition to the basal medium. With this the efficiency of the cell culture can be maintained or even increased while reducing the amount of spent culture medium.
Claims16
A process for perfusion cell culture, comprising culturing cells in a bioreactor system comprising a bioreactor with a media inlet and a harvest outlet whereby
- i. continuously or one or several times during the cell culture process new basal cell culture medium is inserted into the bioreactor via the media inlet
- ii. continuously or one or several times during the cell culture process harvest is removed from the bioreactor via the harvest outlet
- iii. one or several times during the cell culture process a concentrated medium supplement comprising at least phosphotyrosine is inserted into the bioreactor via the media inlet or an additional inlet.
A perfusion cell culture medium kit comprising a basal cell culture medium and a concentrated medium supplement comprising at least phosphotyrosine.
Dependencies are inferred from the claim text.