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US 2026/0286269 A1Published application
Manufacturing method with feedback control
AssigneeChr. Hansen A/S
InventorsKarsten HELLMUTH, Bastian BARTLING, Philipp Paul GRUENERT
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Abstract
The present disclosure generally relates to a method of manufacturing biological product using fermentation and subsequent purification of the biological product, such as an enzyme. By utilization of online biomass data for a feedback control in the fermenter, an efficient and stable cross-flow microfiltration operation can be performed with active cells.
Claims5
A process for producing a product by fermentation of a host cell, said process comprising the steps of:
- a) adding host cell culture to a fermenter;
- b) feeding water or buffer to the fermenter from a buffer tank, operably connected to the fermenter through a pump or valve;
- c) feeding fermentate from the fermenter to a cross-flow microfiltration unit;
- d) feeding biomass back to the fermenter from the cross-flow microfiltration unit;
- and
- e) feeding permeate from the cross-flow microfiltration unit to a product vessel;
- wherein a biomass probe is in the fermenter, said biomass probe measuring cell concentration a process control system receives data about cell concentration and controls the pump or valve feeding water or buffer into the fermenter so that a certain predetermined cell concentration is maintained.
4 more elements
Dependencies are inferred from the claim text.