Systems and methods for cell culturing

A cell culture system, the system comprising:

• a plurality of shelves configured to receive a fluid reservoir;
• at least one pump;
• a processor operably coupled to the at least one pump;
• a platform supporting a substrate comprising a plurality of openings sized and configured to releasably receive and retain at least two interchangeable cell culture chambers in a respective position onto said platform;
• and
• and a central processing unit (CPU) comprising memory containing instructions executable by the CPU to cause the system to:
• receive, as a first input, data comprising a capacity of a first and/or a second cell culture chamber;
• receive, as a second input, data comprising a first concentration of a first cell type and a second concentration of a second cell type in one or more fluids to be introduced into the first and/or second cell culture chamber;
• and
• calculate, based on the first and second inputs, a perfusion rate of a perfusion fluid to be introduced into the first and/or second cell culture chamber that maximizes a probability of the first cell type and the second cell type contacting each other within the cell culture chamber.