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US 2026/0286065 A1Published application
Programming Protein Polymerization with DNA
AssigneeNorthwestern University
InventorsChad A. Mirkin, Janet R. McMillan, Oliver G. Hayes
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Abstract
The present disclosure is generally directed to methods for making protein polymers. The methods comprise utilizing oligonucleotides for controlling the association pathway of oligonucleotide-functionalized proteins into oligomeric/polymeric materials.
Claims41
A method of making a protein polymer comprising contacting:
- (a) a first protein monomer comprising a first protein to which a first oligonucleotide is attached, the first oligonucleotide comprising a first domain (V) and a second domain (W);
- and
- (b) a second protein monomer comprising a second protein to which a second oligonucleotide is attached, the second oligonucleotide comprising a first domain (V′) and a second domain (W′),
- wherein (i) V is sufficiently complementary to V′ to hybridize under appropriate conditions and (ii) W is sufficiently complementary to W′ to hybridize under appropriate conditions, and wherein the contacting results in V hybridizing to V′,
- thereby making the protein polymer.
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A method of making a protein polymer comprising contacting:
- (a) a first protein monomer comprising a first protein to which a first oligonucleotide is attached, the first oligonucleotide comprising a first domain (X), a second domain (Y′), a third domain (Z), and a fourth domain (Y), wherein Y is sufficiently complementary to Y′ to hybridize under appropriate conditions to produce a first hairpin structure;
- (b) a second protein monomer comprising a second protein to which a second oligonucleotide is attached, the second oligonucleotide comprising a first domain (Y), a second domain (X′), a third domain (Y′), and a fourth domain (Z′), wherein Y is sufficiently complementary to Y′ to hybridize under appropriate conditions to produce a second hairpin structure;
- and
- (c) an initiator oligonucleotide comprising a first domain (Y) and a second domain (X′);
- wherein the contacting results in (i) X′ of the initiator oligonucleotide hybridizing to X of the first oligonucleotide and Y of the initiator oligonucleotide displacing Y of the first oligonucleotide, thereby opening the first hairpin structure and (ii) Z′ of the second oligonucleotide hybridizing to Z of the first oligonucleotide thereby opening the second hairpin structure, and
- thereby making the protein polymer.
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Dependencies are inferred from the claim text.