A method for purification of extracellular vesicles or enveloped viruses by means of size exclusion chromatography
AssigneeCytiva BioProcess R&D AB
InventorsJon Lundqvist, Peter Guterstam, Inger Salomonsson
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Abstract
A method of purifying extracellular vesicles or enveloped viruses is provided. The method comprises obtaining a solution volume comprising extracellular vesicles or enveloped viruses and contaminants, adding the solution volume to a size exclusion chromatography column comprising a stationary phase comprising a packed bed of cross-linked polysaccharide beads, and collecting an eluate volume comprising the extracellular vesicles or enveloped viruses exiting the column. Advantageously, the method is scalable and can be used in large scale or industrial scale processes.
Claims15
Method of purifying extracellular vesicles or enveloped viruses, comprising:
- obtaining a solution volume comprising extracellular vesicles or enveloped viruses and contaminants,
- adding the solution volume to a size exclusion chromatography column comprising a stationary phase comprising a packed bed of cross-linked polysaccharide beads,
- collecting an eluate volume comprising the extracellular vesicles or enveloped viruses exiting the column.
Dependencies are inferred from the claim text.