Method of forming multi-specific antibodies from homodimer antibodies and pharmaceutical product including multi-specific antibodies thus obtained

Abstract

The invention relates to a method of forming multi-specific antibodies from first and second homodimer antibodies comprising the steps of: —providing at least one solution including the homodimer antibodies; —loading said at least one solution onto an adsorbent chromatography medium, whereby the homodimer antibodies bind to the medium; —contacting the bound antibodies with a reductant; and —eluting the bound antibodies with an elution buffer thereby obtaining an eluate pool. The method further comprises, after the step of contacting the bound antibodies with a reductant, a step of contacting the bound or eluted antibodies with an oxidant, whereby multi-specific antibodies are formed from first and second homodimer antibodies. The invention is also directed to a pharmaceutical product including multi-specific antibodies obtained by such a method.

Dependencies are inferred from the claim text.