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US 2026/0284667 A1Published application
A method and system for amplification of nucleic acids using polymerase chain reaction (pcr)
AssigneeMerck Patent GmbH
InventorsCamille Correia, Kai Jaehrling, Philipp Lau, Ludger Beylage
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Abstract
The disclosure herein relates to a system for large-scale production of nucleic acids using polymerase chain reaction (PCR) and methods of use thereof.
Claims30
A system for performing polymerase chain reaction (PCR) comprising:
- a) a reaction vessel comprising capillary tubing wound around a frame;
- b) a circulation device for circulating a reaction mixture through the reaction vessel;
- c) at least two temperature-controlled baths holding a fluid for thermal transfer to the reaction mixture;
- and
- d) at least one manipulator to transfer the reaction vessel among the temperature-controlled baths while performing PCR.
2 more elements
The method of claim_20, wherein the product is produced in a batch size from 1 mL to 5 L.
The method of claim_20, further comprising prior to the filling step calculating the diameter and length of the capillary tubing based on the volume of a batch.
Dependencies are inferred from the claim text.