Methods for enclosing and analyzing a cell in a fluidic device
AssigneeCellanome, Inc.
InventorsTarun Kumar KHURANA, Ali AGAH, Yir-Shyuan WU, Filiz Gorpe YASAR, Pier Federico GHERARDINI
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Abstract
The invention is directed to methods and systems for carrying out one or more highly multiplexed cellular assays. In some embodiments, one or more channels of a fluidic device are provided with photopolymerizable polymer precursors and cells randomly disposed on a surface, after which positions of cells are measured by a detector, hydrogel chambers are synthesized by photopolymerization to enclose individual cells, and channels are loaded with assay reagents. Assay signals indicative of desired cellular characteristics can be generated for each of the enclosed cells.
Claims28
A method for cellular analysis, comprising:
- (a) disposing a plurality of cells adjacent to a surface, wherein:
- the surface comprises a plurality of capture sites,
- the plurality of capture sites have a density from about 10 to about 2500 reaction sites per square millimeter on at least a portion of the surface,
- the plurality of capture sites comprise a plurality of capture oligonucleotides, and
- a density of capture oligonucleotides within a capture site of the plurality of capture sites is at least about 1×1012 capture oligonucleotides per square centimeter;
- (b) releasing nucleic acids from at least a subset of the plurality of cells;
- and
- (c) capturing at least a subset of the nucleic acids on at least a subset of the capture oligonucleotides.
Dependencies are inferred from the claim text.