Multiple port pipette tip
AssigneeScientific Plastic Products, Inc.
InventorsSamuel A. Ellis, Elio Landaverde, Joseph S. Caustill, Kishan G. Hingorani
Life of this patent
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Abstract
The disclosed multiple port pipette tip comprises four port adapters releasably engageable automated pipette system shafts. The adapters each include gas permeable membranes and are collectively connected to an elongate tubular body to form a fluid cavity and an end opening to form a pipette tip to engage a sample fluid. The gas permeable membranes prevent sample liquid from contaminating the pipette system shafts. The adapters allow the multiple port pipette tip to draw, retain, and dispense a greater volume than that of a standard single port pipette tip.
Claims20
A multiple port pipette tip comprising:
- an elongate tubular body having a proximal end having an opening and a distal tapered end having an opening;
- a platform having a proximal face and opposed distal face and at least one opening formed in the platform, wherein the distal face is coupled to the proximal end of the tubular body such that the at least one opening is in fluid communication with the opening of the distal end of the tubular body;
- at least two generally cylindrical protrusions, wherein the protrusions have a proximal end having an opening and a distal end having an opening wherein the distal ends are coupled to the proximal face such that the openings of the distal ends of the protrusions are in fluid communication with the platform opening;
- and
- a membrane positioned within each of the at least two generally cylindrical protrusions between the proximal end opening and the distal end opening of the at least two cylindrical protrusions.
A method of pipetting a fluid sample comprising:
- forming a multiple port pipette tip comprising:
- an elongate tubular body having a proximal end having an opening and a distal tapered end having an opening, the interior of the elongate tubular body between the opening of the proximal end and the opening of the distal end comprising a fluid cavity;
- a platform having a proximal face and opposed distal face and at least one opening formed in the platform, wherein the distal face is coupled to the proximal end of the tubular body such that the at least one opening is in fluid communication with the opening of the distal end of the tubular body;
- at least two generally cylindrical protrusions, wherein the protrusions have a proximal end having an opening and a distal end having an opening wherein the distal ends are coupled to the proximal face such that the openings of the distal ends of the protrusions are in fluid communication with the platform opening, the proximal end being adapted to be coupled to a pipetting shaft of a pipetting system;
- and
- positioning a membrane within each of the at least two generally cylindrical protrusions between the proximal end opening and the distal end
- releasably coupling each of the at least two generally cylindrical protrusions to one of at least two pipetting shafts of a pipetting system;
- submerging the distal tapered end opening of the elongate tube of the multiple port pipette tip into a fluid sample contained in a first vessel;
- drawing the fluid sample into the fluid cavity of the elongate tubular body using negative pressure of the pipetting system;
- transporting the fluid sample within the fluid cavity to a second vessel;
- and
- dispensing the fluid sample from the fluid cavity into the second vessel using positive pressure of the pipetting system.
Dependencies are inferred from the claim text.