Electroporation/electrochemotherapy of internal regions with low infected depth

A system for in-vivo electroporation of a plurality of target cells in an internal target region of a living body, the system comprising:

• a tubular probe configured to transfer a pulsed electric field to the plurality of target cells, the tubular probe comprising:
• a support tube comprising a plurality of openings thereon, the support tube comprising a distal end and a proximal end, the distal end of the support tube configured to be inserted into the internal target region via guiding the proximal end;
• a plurality of circumferential rings defining a first set of electrodes, the plurality of circumferential rings positioned around the support tube in an axially spaced apart relation from each other along the support tube, the first set of electrodes configured to be put in contact with the internal target region;
• a head part attached to the distal end of the support tube, the head part comprising:
• an electrode substrate engaged to the distal end of the support tube, the electrode substrate comprising at least two apertures;
• and
• at least two U-shaped wires protruded out from the respective at least two apertures, the at least two U-shaped wires defining a second set of electrodes configured to be put in contact with the internal target region;
• and
• a plurality of electrical connectors passing through the support tube, the plurality of electrical connectors comprising:
• a first set of electrical connectors, each respective electrical connector of the first set of electrical connectors comprising an electrically conductive line comprising a distal end and a proximal end, the respective distal end passed through a respective opening of the plurality of openings and attached to a respective circumferential ring of the plurality of circumferential rings, the distal end of the electrically conductive line fastening the respective circumferential ring around the support tube;
• and
• a second set of electrical connectors, each respective electrical connector of the second set of electrical connectors comprising an electrically conductive line comprising a distal end and a proximal end, the respective distal end attached to a respective U-shaped wire of the at least two U-shaped wires,
• an electrical pulse generator configured to apply a pulsed electric field between at least two electrodes of at least one of the first set of electrodes, the second set of electrodes, and combinations thereof, each respective proximal end of each electrically conductive line of the at least two electrodes being connected to a different pole of two poles of the electrical pulse generator;
• and
• a processing unit electrically connected to the electrical pulse generator, the processing unit comprising:
• a memory having processor-readable instructions stored therein;
• and
• a processor configured to access the memory and execute the processor-readable instructions, which, when executed by the processor configures the processor to perform a method, the method comprising:
• inducing electroporation to the plurality of target cells by generating the pulsed electric field between the at least two electrodes of at least one of the first set of electrodes, the second set of electrodes, and combinations thereof inside the internal target region via applying at least one sequence of electric voltage pulses between the at least two electrodes utilizing the electrical pulse generator.