Method for producing human acellular dermal matrix
AssigneeCGBio Co., Ltd.
InventorsHong Sun YOON, Jiwon WOO, Kwon Young LEE
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Abstract
The present invention relates to a method for producing a human acellular dermal matrix, wherein secondary washing is performed with an alcohol-based delipidation solution to provide excellent safety with respect to cytotoxicity and effectively remove residual fat. An object of the present disclosure is to provide a method for preparing the human body-derived acellular dermal matrix capable of effectively removing fat remaining in the dermis.
Claims13
A method for preparing a human body-derived acellular dermal matrix, comprising steps of:
- (i) preparing a human body-derived skin tissue on which separate de-epidermization and delipidation processes have not been performed;
- (ii) immersion-treating the skin tissue in a hypotonic solution containing a surfactant;
- (iii) primarily washing the skin tissue that has been immersion-treated in the step (ii) with an isotonic solution;
- and
- (iv) secondly washing the skin tissue that has primarily been washed in the step (iii) with a delipidation solution.
A human body-derived acellular dermal matrix prepared by washing multiple times a human body-derived skin tissue on which separate de-epidermation and delipidation processes have not been performed, wherein the washing includes washing with an isotonic solution and washing with a delipidation solution, and the delipidation solution comprises a polar solvent.
Dependencies are inferred from the claim text.