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US 2026/0284175 A1Published application
Hiv immunogenic polypeptides and vaccines and uses thereof
AssigneeGilead Sciences, Inc.
InventorsElena Bekerman, Jiani Li, Stephen R. Martin, Devi SenGupta, Evguenia Svarovskaia
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Abstract
The present disclosure relates HIV immunogenic polypeptides, polynucleotides encoding such HIV immunogenic polypeptides, self-amplifying messenger RNA (SAM) vectors comprising such polynucleotides, and viral vectors comprising such polynucleotides.
Claims179
A self-amplifying RNA (SAM or saRNA) comprising:
- a. a polynucleotide comprising any one of the nucleic acid sequences of SEQ ID NOS:
- 522-523;
- b. a polynucleotide comprising a nucleic acid sequence that is at least 80%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or 100% identical to any one of the nucleic acid sequences of SEQ ID NOs:
- 522-523, wherein percent identity is across the full length of the nucleic acid sequence of SEQ ID NOs:
- 522-523;
- c. a polynucleotide comprising any one of the nucleic acid sequences of SEQ ID NOs:
- 524-526;
- d. a polynucleotide comprising a nucleic acid sequence that is at least 80%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or 100% identical to any one of the nucleic acid sequences of SEQ ID NOs:
- 524-526, wherein percent identity is across the full length of the nucleic acid sequence of SEQ ID NOs:
- 524-526;
- e. a polynucleotide that encodes any one of the amino acid sequences of SEQ ID NOs:
- 527-528;
- f. a polynucleotide that encodes a polypeptide that is at least 80%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or 100% identical to any one of the amino acid sequences of SEQ ID NOs:
- 527-528, wherein percent identity is across the full length of the amino acid sequence of SEQ ID NOs:
- 527-528;
- g. (i) a first polynucleotide sequence comprising nucleotides 1 to 7512 of SEQ ID NO:
- 522;
- (ii) a promoter sequence;
- (iii) a second polynucleotide sequence comprising nucleotides 7537 to 7570 of SEQ ID NO:
- 522;
- (iv) a third polynucleotide sequence that encodes a polypeptide comprising an amino acid sequence that is at least 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98% or at least 99% identical to the amino acid sequence of any one of SEQ ID NOs:
- 345-371, 373-377, 407-411, 422-423, 430-435, 527, and 528, wherein percent identity is across the full length of SEQ ID NOs:
- 345-371, 373-377, 407-411, 422-423, 430-435, 527, and 528;
- (v) a fourth polynucleotide sequence comprising nucleotides 10667 to 10952 of SEQ ID NO:
- 522;
- and (vi) a polyA region;
- or
- h. (i) a first polynucleotide sequence comprising nucleotides 1 to 7512 of SEQ ID NO:
- 522;
- (ii) a promoter sequence;
- (iii) a second polynucleotide sequence comprising nucleotides 7537 to 7570 of SEQ ID NO:
- 522;
- (iv) a third polynucleotide sequence that encodes one or more polypeptide segments, wherein the one or more polypeptide segments comprise an amino acid sequence that is at least 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98% or at least 99% identical to the amino acid sequence of any one of SEQ ID NOs:
- 1-344, wherein percent identity is across the full length of SEQ ID NOs:
- 1-344;
- (v) a fourth polynucleotide sequence comprising nucleotides 10667 to 10952 of SEQ ID NO:
- 522;
- and (vi) a polyA region.
35 more elements
An expression vector comprising:
- a. any one of the nucleic acid sequences of SEQ ID NOs:
- 524-526;
- b. a nucleic acid sequence that is at least 80%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or 100% identical to any one of the nucleic acid sequences of SEQ ID NOs:
- 524-526, wherein percent identity is across the full length of the nucleic acid sequence of SEQ ID NOs:
- 524-526;
- c. a polynucleotide sequence that encodes for any one of the amino acid sequences of SEQ ID NOs:
- 527-528;
- d. a polynucleotide sequence that encodes for polynucleotide that is at least 80%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or 100% identical to any one of the amino acid sequences of SEQ ID NOs:
- 527-528, wherein percent identity is across the full length of the amino acid sequence of SEQ ID NOs:
- 527-528,
- e. (i) a first polynucleotide sequence comprising nucleotides 1 to 593 of SEQ ID NO:
- 520;
- (ii) a promoter sequence;
- (iii) a second polynucleotide sequence comprising nucleotides 1196 to 1255 of SEQ ID NO:
- 520;
- (iv) a third polynucleotide sequence encodes a polypeptide comprising an amino acid sequence that is at least 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98% or at least 99% identical to the amino acid sequence of any one of SEQ ID NOs:
- 345-371, 373-377, 407-411, 422-423, 430-435, 527, and 528, wherein percent identity is across the full length of SEQ ID NOs:
- 345-371, 373-377, 407-411, 422-423, 430-435, 527, and 528;
- (v) a fourth polynucleotide sequence comprising nucleotides 4352 to 4395 of SEQ ID NO:
- 520;
- (vi) a polyA sequence;
- and (vii) a fifth polynucleotide sequence comprising nucleotides 4620 to 32350 of SEQ ID NO:
- 520;
- or
- f. (i) a first polynucleotide sequence comprising nucleotides 1 to 593 of SEQ ID NO:
- 520;
- (ii) a promoter sequence;
- (iii) a second polynucleotide sequence comprising nucleotides 1196 to 1255 of SEQ ID NO:
- 520;
- (iv) a third polynucleotide sequence that encodes one or more polypeptide segments, wherein the one or more polypeptide segments comprise an amino acid sequence that is at least 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98% or at least 99% identical to the amino acid sequence of any one of SEQ ID NOs:
- 1-344, wherein percent identity is across the full length of SEQ ID NOs:
- 1-344;
- (v) a fourth polynucleotide sequence comprising nucleotides 4352 to 4395 of SEQ ID NO:
- 520;
- (vi) a polyA sequence;
- and (vii) a fifth polynucleotide sequence comprising nucleotides 4620 to 32350 of SEQ ID NO:
- 520.
34 more elements
A polypeptide comprising:
- a. any of the HIV immunogen amino acid sequences of SEQ ID NOs:
- 527-528;
- or
- b. an amino nucleic acid sequence that is at least 80%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or 100% identical any of the HIV immunogen amino acid sequences of SEQ ID NOs:
- 527-528, wherein percent identity is across the full length of the HIV immunogen amino acid sequence of SEQ ID NOs:
- 527-528.
3 more elements
Dependencies are inferred from the claim text.