Recombinant expression vector for production of virus-like particle-based multivalent norovirus vaccine and manufacturing method therefor

Abstract

The present invention provides a fusion protein for promoting soluble expression of a norovirus antigen, and an expression vector thereof. More specifically, the present invention provides combinations of various genotypes of norovirus antigen proteins and RID mutants, in which the most efficient folding occurs when a norovirus antigen is used as a target protein to form a recombinant fusion protein, the yield of soluble expression of a fusion protein produced is improved, and the assembly efficacy and homogeneity of norovirus VLPs can be enhanced. Also, the present invention provides a method for rapidly mass-producing various other genotypes of norovirus VLPs, as well as GII.4, in E. coli by using mutant RID, thus enabling the development of a VLP-based multivalent vaccine comprising more genotypes.

Dependencies are inferred from the claim text.