Therapeutics and methods

A ion channel modulator for use in organ regeneration or repair, wherein the ion channel modulator reduces ion flow through sodium leak channel (NALCN).

The modified stem cell-like cell or ion channel modulator for use according to any preceding claim wherein the organ to be regenerated is selected from:

• thymus, adrenal gland, thyroid gland, intestine, lungs, heart, liver, blood vessels, germ cells, nervous system, eye tissues, hair cells, kidney and bladder, skin, hair follicles, pancreas, bone, and cartilage.

The modified stem cell-like cell or ion channel modulator for use according to any preceding claim, wherein the reduced ion flow through NALCN is transient.

A method of preparing a modified stem cell-like cell the method comprising:

• i) contacting a stem cell-like cell with an ion channel modulator, wherein the ion channel modulator inhibits ion flow through NALCN;
• or
• ii) introducing a mutation in and/or deleting Nalcn and/or reducing the expression of Nalcn in a stem cell-like cell, wherein the mutation and/or deletion and/or reduced expression results in reduced ion flow through NALCN.

A method of increasing the level of solid tissue cell shedding of stem cell-like cells comprising introducing a mutation in and/or deleting Nalcn and/or reducing the expression of Nalcn in a stem cell-like cell, wherein the mutation and/or deletion and/or reduced expression results in reduced ion flow through NALCN.

A method of increasing the level of solid tissue cell shedding of stem cell-like cells comprising contacting a cell with an ion channel modulator wherein said ion channel modulator reduces ion flow through NALCN.

A kit comprising a modified stem cell-like cell, wherein the modified stem cell-like cell exhibits reduced ion flow through NALCN and optionally instructions for use.

A kit comprising a means to obtain a cell sample from a subject and components to modify a cell to reduce ion flow through NALCN.