Methods and materials for organ perfusion

A method of preserving a donor organ, the method comprising:

• combining one or more of a mineralocorticoid receptor antagonist (MRA) and a Brd4-targeting compound with HTK solution to generate a first solution;
• perfusing the donor organ with the first solution using cold perfusion techniques at a temperature of about 2° C. to about 15° C.;
• perfusing the donor organ with a second solution, wherein the donor organ is continuously perfused with the second solution for an amount of time prior to transplant to a recipient subject;
• and
• preparing the donor organ for transplantation to the recipient subject.

A method of extending donor organ viability for transplantation to a recipient subject, the method comprising:

• positioning a donor organ in a receptacle comprising a first solution comprising one or more of a mineralocorticoid receptor antagonist (MRA) and a Brd4-targeting compound;
• perfusing the donor organ with the first solution for a first amount of time;
• removing the first solution from the donor organ;
• contacting the donor organ with a second solution for a second amount of time;
• and
• preparing the donor organ for transplantation to the recipient subject after the second amount of time.