Method for gender determination of chicken embryos
AssigneeEggxyt Ltd.
InventorsDaniel Yehuda OFFEN, Yehuda ELRAM, Eliran KADOSH, Malkiel COHEN
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Abstract
The present invention provides a transgenic female chicken, including a recombinant Z gender chromosome including a nucleic acid sequence of an exogenous reporter gene encoding a red fluorescent protein (RFP), in at least one location of the Z gender chromosome. Further provided is a method of gender determination of a chicken embryo in an unhatched egg.
Claims22
A transgenic female chicken comprising a recombinant Z gender chromosome comprising a nucleic acid sequence of an exogenous reporter gene encoding a red fluorescent protein (RFP), in at least one location of a Z gender chromosome of a female chicken, wherein said at least one location is listed under Table 1.
(canceled)
A kit comprising at least one first nucleic acid molecule encoding a guide RNA (gRNA), comprising the nucleotide sequence as set forth in any one of SEQ ID Nos:
- 1-3, and 18-20.
A method for producing a chicken comprising a recombinant Z gender chromosome, the method comprising:
- (a) obtaining at least one transgenic PGC comprising a recombinant Z chromosome comprising a nucleic acid sequence of an exogenous reporter gene encoding an RFP in at least one location as listed under Table 1 in Z gender chromosome of a chicken;
- and,
- (b) transplanting said transgenic PGC into a receptive chicken embryo,
- thereby producing a chicken comprising the recombinant Z gender chromosome.
(canceled)
Dependencies are inferred from the claim text.