Single-cell manipulation device and methods operating the same

Abstract

Disclosed is a single-cell manipulation device, including a stage, a probe, a probe control assembly, a microscopic imaging assembly, and a pneumatic pressure source assembly. The pneumatic pressure source assembly is configured to provide positive and/or negative pneumatic pressure to the at least one probe in the form of pulses. A method for extracting single cells, a method for injecting a reagent into a single cell and/or extracting contents from a single cell, and a method for performing sample spotting using the single-cell manipulation device are further disclosed. By providing pulsed positive and negative pneumatic pressure to the probe, the present disclosure not only enables precise in-situ extraction of target cells, but also allows the extracted cells to enter the interior of the probe. Thus, the probe serves as a temporary storage site for cells, enabling continuous extraction of multiple cells and improving operational efficiency.

Dependencies are inferred from the claim text.