Determination of progestogens non-invasively in animals
AssigneeVetcare Oy
InventorsSari VIITALA, Tia PENNANEN
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Abstract
The present disclosure relates to 5α-pregnane-3,6,20-triol isomer or 5α-pregnane-3,6,20-triol in a glucuronidated form in determination of progestogen or progestogens in a sample from a non-human animal. The present disclosure also relates to an in vitro method of determining progestogen or progestogens in a sample from a non-human animal.
Claims23
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Use of a 5α-pregnane-3,6,20-triol isomer or 5α-pregnane-3,6,20-triol glucuronide isomer in determination of progestogen or progestogens in an in vitro urine sample from a non-human animal.
An in vitro method of determining progestogen or progestogens in a urine sample from a non-human animal, characterized in that the method comprises the steps of
- providing an in vitro urine sample from a non-human animal,
- measuring an amount of 5α-pregnane-3,6,20-triol isomer or 5α-pregnane-3,6,20-triol glucuronide isomer in said sample,
- determining the presence of luteinized follicular theca and/or granulosa cells based on the amount of 5α-pregnane-3,6,20-triol isomer or 5α-pregnane-3,6,20-triol glucuronide isomer in the sample,
- wherein the progestogen level in the sample is indicative by the presence of luteinized follicular theca and/or granulosa cells.
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Dependencies are inferred from the claim text.