Method and kit for the diagnosis of oral carcinoma
OwnerRiccardo MOFFA, Angelo Simone PARODI +1inventors
InventorsRiccardo MOFFA, Angelo Simone PARODI, Emanuela MARCENARO
Life of this patent
Estimating the term…
Abstract
A method and kit are used to analyze the protein levels of EGFR, p53, B7H6, PdL1 in a sample from a subject and estimate the presence of oral squamous cell carcinoma in the subject. The method and kit compare the protein levels in the sample with levels of corresponding proteins in samples isolated from control individuals and/or with levels recorded in reference databases.
Claims14
An in vitro method for the diagnosis and/or prognosis of oral squamous cell carcinoma comprising the following steps:
- providing an isolated sample from an individual,
- analyzing protein levels of EGFR, p53, B7H6, PdL1, in the sample,
- comparing the protein levels in the sample with levels of corresponding proteins in samples isolated from control individuals and/or with levels recorded in reference databases,
- wherein an alteration, in terms of increase, of the levels of the proteins in the sample is indicative of a presence of oral squamous cell carcinoma in the individual.
A kit for diagnosis and/or prognosis of oral squamous cell carcinoma comprising:
- a device suitable for taking a sample of an oral cavity containing cells, preferably a cytobrush,
- two series of primary antibodies, preferably one series of monoclonals and one series of polyclonals, anti-EGFR, anti-p53, anti-B7H6, anti-PdL1 E′ MEGLIO ANTI o AGAINST? from two different animal species,
- a signal detection and/or quantification system comprising a secondary antibody, conjugated with a detection compound, preferably alkaline phosphatase or peroxidase, directed against one of two animal species from which the primary antibodies come
- and optionally instructions for use.
Dependencies are inferred from the claim text.