Assays for characterization of extracellular vesicles and methods of using the same
AssigneesThe Translational Genomics Research Institute, City of Hope, The General Hospital Corporation
InventorsTijana Talisman, Kendall Jensen, Andras Saftics, Saumya Das
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Abstract
Disclosed herein, in certain embodiments, are methods for isolation and characterization of extracellular vesicles (EVs) subpopulations derived from one or more target tissues of interest using a novel assay that combines affinity capture of EVs, super-resolution microscopy, transcriptomic analysis, and proteomic analysis.
Claims72
A multiplexed method for characterizing a population of tissue-specific extracellular vesicles (TSEVs) in a biofluid and/or tissue sample, comprising a mixed population of EVs (MEVs), comprising:
- (a) immobilizing the population of TSEVs in the sample on a surface functionalized with an affinity capture agent that specifically binds to a biomarker present on the plasma membrane of TSEVs;
- and
- (b) analyzing individual TSEVs within the population TSEVs using super-resolution microscopy to produce a profile of the population of TSEVs;
- wherein the profile of the population of TSEVs includes information on at least:
- (i) membrane protein composition of individual TSEVs;
- and
- (ii) intra-vesicular cargo composition of individual TSEVs.
Dependencies are inferred from the claim text.