Apparatus and method for the capture of intracelluar activity with nanofids

A nanofid apparatus system, comprising in combination:

• a plurality of nanofid apparatus including:
• a photovoltaic panel (PV) layer, including a photoelectric panel with a plurality of different layers, powered by near infrared (NIR) light from a NIR light source apparatus external to the plurality nanofid apparatus, configured for providing power including an electrical current to the plurality nanofid apparatus,
• a quantum dots (QD) light emitting diodes (LEDs) layer, including a plurality of QD-LEDs, configured for emitting NIR light at a plurality of different wavelengths when activated by a Field Electric Transistor (FET) layer and a molecule sensor layer,
• a logic layer, including a pulse comparator, configured for comparing electrical current pulses generated from the PV layer with strobed light using strobed input coding and activating one or more of the plurality of QD-LEDs when the electrical current increases and exceeds a pre-determined electrical current threshold,
• the Field Electric Transistor (FET) layer, including an electrical biosensor field-effect transistor with a transistor gate and a graphene layer, configured for detecting changes in a surface potential of the graphene layer induced by binding of pre-determined intracellular molecules on the molecule sensor layer,
• the molecule sensor layer, including a plurality of molecule sensors for sensing concentrations of the pre-determined intracellular molecules in a pre-determined biological tissue, configured for sensing changes in electrical charges of the concentrations of the pre-determined intracellular molecules detected on a surface of graphene layer that alters electrical current through the graphene layer sensing concentrations of the pre-determined intracellular molecules in the pre-determined biological tissue, and
• a silicon carbide (SiC) coating, including an inert coating biocompatible for a plurality of different pre-determined types of biological tissue, configured for coating and protecting the photovoltaic panel (PV) layer, QD-LED layer, logic layer and FET layer of the plurality of nanofid apparatus within the pre-determined biological tissue,
• wherein the plurality of the nanofid apparatus are inserted directly into a plurality of individual cells in the pre-determined biological tissue for sensing concentrations of the pre-determined intracellular molecules in the plurality of individual cells in the pre-determined biological tissue, without causing any harm, toxicity or death to the plurality of individual cells in the pre-determined biological tissue or the pre-determined biological tissue;
• the NIR light source apparatus external to the plurality of nanofid apparatus, configured for providing NIR light at a plurality of different wavelengths via optical fibers external to the plurality of nanofid apparatus;
• an nanofid optical collection system external to the plurality nanofid apparatus, configured for collecting output NIR light data from the NIR light at the plurality of different wavelengths emitted from the plurality of QD-LEDs on the plurality of nanofid apparatus inserted into the plurality of individual cells in the pre-determined biological tissue, the output NIR light data received from the plurality of QD-LEDs activated by the FET layer and the molecule sensing layer sensing concentrations of the pre-determined intracellular molecules in the plurality of individual cells in the pre-determined biological tissue;
• a nanofid signal processing system external to the plurality of nanofid apparatus, configured for processing the output NIR light data collected by the optical collection system with one or more signal processing methods and creating signal processing data with the one or more signal processing methods;
• a nanofid data processing system external to the plurality of nanofid apparatus, configured for analyzing the created signal processing data from the signal processing system and producing and displaying an output report for sensing concentrations of the pre-determined intracellular molecules in the plurality of individual cells in the pre-determined biological tissue sensed by the plurality of nanofid apparatus inserted into the plurality of individual cells in the pre-determined biological tissue;
• and
• a nanofid injection system external to the plurality of nanofid apparatus, including an injection apparatus and an injection process configured for injecting the plurality of nanofids apparatus directly into the plurality of individual cells in the pre-determined biological tissue without causing any harm, toxicity or death to the plurality of individual cells in the pre-determined biological tissue or the pre-determined biological tissue during the injection process.

A method for determining intracellular activity with nanofid apparatus, comprising:

• providing a plurality of nanofid apparatus that are completely inserted into a plurality of cells, the plural nanofid apparatus including a plurality of functional layers for detecting a biological substance of interest in the plurality of cells;
• providing a nanofid optical collection system with an optical fiber array external to the plurality of nanofid apparatus;
• providing a Near Infrared (NIR) light source external to the plurality of nanofid apparatus to provide a power source to the plurality of nanofid apparatus in the plurality of cells with NIR light;
• providing one or more signal processing methods to process inputs and outputs from the plurality of nanofid apparatus in the plurality of cells, the one or more signal processing methods including processing inputs made from the NIR light source to activate the plurality of nanofid apparatus in the plurality of cells and processing outputs with emissions of NIR light from the plurality of nanofid apparatus including a plurality of unique cell identifiers when the biological substance of interest is detected in the plurality of cells with the nanofid optical collection system;
• inserting the plurality of nanofid apparatus inside the plurality of cells;
• detecting the biological substance of interest in the plurality of cells with the plurality of nanofid apparatus in the plurality of cells with the nanofid optical collection system with the optical fiber array external to the plurality of nanofid apparatus and the one or more signal processing methods;
• collecting information about the biological substance of interest in the plurality of cells with the plurality of nanofid apparatus with the nanofid optical collection system with the optical fiber array and one or more different hardware devices external to the plural nanofid apparatus and the one or more signal processing methods;
• processing the collected information uniquely identifying and recording all cell activity for the biological substance of interest in the plurality of cells to the individual cell level with a plurality of different hardware devices to create digital cell activity information about the plurality of cells;
• processing the collected digital cell activity information from the one or more different hardware devices uniquely identifying and recording all cell activity for the biological substance of interest in the plurality of cells to the individual cell level with one or more applications on one or more network devices, each with one or more processors to create an output report;
• and
• displaying on the output report on the one or more applications on the one or more network devices uniquely identifying and recording all cell activity for the biological substance of interest in the plurality of cells to the individual cell level.

A method for determining intracellular activity with nanofid apparatus, comprising:

• injecting a plurality of nanofid apparatus into a plurality of individual cells in a pre-determined tissue type with a nanofid injection system;
• applying input Near InfraRed (NIR) light from a NIR light source apparatus external to the plurality of nanofid apparatus, via a plurality of optical fibers external to the plurality of nanofid apparatus to provide power and activate the plurality of nanofid apparatus in the plurality of cells in the pre-determined tissue type;
• collecting on a nanofid optical collection system external to the plurality nanofid apparatus output NIR light data at a plurality of different wavelengths emitted the plurality of nanofid apparatus inserted into the plurality of individual cells in the pre-determined biological tissue,
• the output NIR light data received from the plurality of nanofid apparatus sensing concentrations of pre-determined intracellular molecules in the plurality of individual cells in the pre-determined biological tissue;
• processing on a nanofid signal processing system external to the plurality of nanofid apparatus to output NIR light data collected by the optical collection system with one or more signal processing methods and creating signal processing data with one or more signal processing methods;
• creating on a nanofid data processing system external to the plurality of nanofid apparatus an output report from the created signal processing data created by the nanofid signal processing system from sensing concentrations of the pre-determined intracellular molecules in the plurality of individual cells in the pre-determined biological tissue sensed by the plurality of nanofid apparatus inserted into the plurality of individual cells in the pre-determined biological tissue;
• and
• displaying on the nanofid data processing system external to the plurality of nanofid apparatus the created output report created from the created signal processing data created by the nanofid signal processing system from sensing concentrations of the pre-determined intracellular molecules in the plurality of individual cells in the pre-determined biological tissue sensed by the plurality of nanofid apparatus inserted into the plurality of individual cells in the pre-determined biological tissue.