US 2026/0276583 A1Published application

Analysis method and capillary electrophoresis device

AssigneeHitachi High-Tech Corporation

InventorsAsami TERAKADO, Motohiro YAMAZAKI, Hirokazu KATO, Noriyuki SUMIDA, Hiroyuki TANAI

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Abstract

An object of the invention is to provide an analysis method, in which unnecessary re-electrophoresis and sample repreparation are suppressed. To this end, the invention includes a method of analyzing features involved in a sample by electrophoresing the sample using a capillary and a sieving matrix, the method including: a reading step of reading an analysis condition; a sieving matrix filling step of filling the capillary with the sieving matrix; a sample injection step of injecting the sample into the capillary containing the sieving matrix charged in the sieving matrix filling step; an electrophoresing step of electrophoresing the sample in the sieving matrix, the sample being injected in the sample injection step; an abnormality determination step of statistically determining whether an analysis result obtained in the electrophoresing step is abnormal by comparing the analysis result with another analysis result obtained under the same analysis condition; and an analysis condition adjustment step of adjusting the analysis condition, where if the analysis result is determined to be abnormal in the abnormality determination step, the method proceeds to the reading step again after the analysis condition adjustment step is performed.

Claims10

Dependent claims

A method of analyzing features involved in a sample by electrophoresing the sample using a capillary and a sieving matrix, the method comprising:

a reading step of reading an analysis condition;
a sieving matrix filling step of filling the capillary with the sieving matrix;
a sample injection step of injecting the sample into the capillary containing the sieving matrix charged in the sieving matrix filling step;
an electrophoresing step of electrophoresing the sample in the sieving matrix, the sample being injected in the sample injection step;
a signal height determination step of determining whether a signal height detected by a detection unit during application of laser light in the electrophoresing step is less than a predetermined threshold;
an abnormality determination step of statistically determining whether an analysis result obtained in the electrophoresing step is abnormal by comparing the analysis result with another analysis result obtained under the same analysis condition;
and
an analysis condition adjustment step of adjusting the analysis condition,
wherein if the signal height is determined to be less than the predetermined threshold in the signal height determination step, and if the analysis result is determined to be abnormal in the abnormality determination step, in the analysis condition adjustment step, an application condition of a voltage to the capillary by a power supply during injection of the sample or an application condition of the laser light by the detection unit is adjusted to a condition under which intensity of a signal detected by the detection unit increases, and then the method proceeds to the reading step again.

6 more elements

A capillary electrophoresis device, comprising:

a capillary to be filled with a sieving matrix;
a power supply that applies a voltage to the capillary to electrophorese a sample;
and a detection unit that detects a signal when laser is applied to the sample being electrophoresed in the capillary;
and
a control section that controls operations of the power supply and the detection unit, and calculates an analysis result,
wherein the control section:
determines whether a signal height detected by the detection unit is less than a predetermined threshold, and compares the calculated analysis result with another analysis result obtained under the same analysis condition and thus statistically determines whether the calculated analysis result is abnormal;
and
when determining the signal height to be less than the predetermined threshold, and when determining the analysis result to be abnormal, adjusts an application condition of a voltage to the capillary by the power supply during injection of the sample or an application condition of the laser light by the detection unit to a condition under which intensity of the signal detected by the detection unit increases, and operates the power supply and the detection unit under the adjusted analysis condition and performs reanalysis.

6 more elements

Dependencies are inferred from the claim text.