US 2026/0276575 A1 · PEPTIDE BASED BRIDGES FOR MOLECULAR SENSORS AND METHODS FOR USE THEREOF

Abstract

In various embodiments alpha helical peptide-based bridges for molecular biosensing “on-chip” are disclosed. The Peptide-based bridges serve as common bridges for great diversity of biosensing applications and targets including nucleic acids, proteins, antigens, antibodies, small molecules. The primary sensor element is preferably a “molecular wire” typically comprised of 10-45 nm long alpha-helical peptide integrated into a current monitoring circuit. The engineered peptide may contain a central conjugation site for attachment of various probe molecules including nucleic acids, proteins, antigens, antibodies. The probe-containing bridge empowers the sensor to detect interactions with specific target molecules.

Claims20

Dependent claims

A sensor device comprising:

a current carrying molecular structure comprising a metal contact on the surface of an electrode, wherein said metal contact is coupled to a bridge molecule,
wherein the bridge molecule comprises a peptide alpha helix having one or more metal binding motif selected from QQSWPIS (SEQ ID NO:2), CTLHVSSYC (SEQ ID NO:3), TLHVSSY (SEQ ID NO:
4), KGSGKGSGK (SEQ ID NO:5), CCPGCC (SEQ ID NO:6), CPTSTGQA (SEQ ID NO:
7), HFYWM (SEQ ID NO:8), HFYWMR (SEQ ID NO:9), PTSTGQA (SEQ ID NO:10), NFMSLPRLGHMH (SEQ ID NO:11) or any one of the preceding having one or two amino acid deletions or conservative amino acid substitutions;
and
a probe molecule coupled to the bridge molecule;
wherein a circuit is formed that is capable of detecting and/or obtaining detailed information about the binding of the probe to a target ligand or binding partner.

4 more elements

Dependencies are inferred from the claim text.

Facts

Application

18/843,280Filed 1 Mar 2023

Priority

10 Jan 2020

Published

17 Sep 2026

Term ends

Not estimatedThe source records don’t support an estimate.

Classification9 codes

G01N 27/3271
Biochemical electrodes , e.g. electrical or mechanical details for in vitro measurements: amperometric enzyme electrodes for analytes in body…
G01N 33/5438
Involving physiochemical end-point determination, e.g. wave-guides, FETS, gratings: electrodes
C12Q 1/001
Measuring or testing processes involving enzymes, nucleic acids or microorganisms ; Compositions therefor; Processes of preparing such…
C12Q 1/48
Measuring or testing processes involving enzymes, nucleic acids or microorganisms ; Compositions therefor; Processes of preparing such…

Peptide based bridges for molecular sensors and methods for use thereof

Life of this patent

Peptide based bridges for molecular sensors and methods for use thereof

Life of this patent

Abstract

Claims20