Single extracellular vesicle monitoring in crude cultures
OwnerAbigail A. WEAVER, Paul W. BOHN +1inventors
InventorsAbigail A. WEAVER, Paul W. BOHN, Jarek James METRO
Life of this patent
Estimating the term…
Abstract
Described herein is a zero-mode waveguide (ZMW) array for monitoring populations of single extracellular vesicles in a crude culture in real-time. The ZMW array comprises a plurality of ZMW nanopores arranged in parallel, each ZMW nanopore being configured to hold a single extracellular vesicle. Also described herein are methods and kits for monitoring populations of single extracellular vesicles in crude cultures using ZMW arrays.
Claims23
A zero-mode waveguide (ZMW) array for monitoring populations of single extracellular vesicles in a crude culture, the ZMW array comprising a plurality of ZMW nanopores arranged in parallel, each ZMW nanopore being configured to hold a single extracellular vesicle.
A method for monitoring single extracellular vesicles in a crude culture, the method comprising:
- labeling single extracellular vesicles in the crude culture with a lipophilic fluorophore;
- contacting the crude culture with a zero-mode waveguide (ZMW) array comprising a plurality of ZMW nanopores arranged in parallel, each ZMW nanopore being configured to hold a single extracellular vesicle, wherein single extracellular vesicles diffuse into the ZMW nanopores;
- and
- detecting fluorescence from single extracellular vesicles positioned within the ZMW nanopores.
16-18. (canceled)
22-24. (canceled)
A kit for monitoring single extracellular vesicles in a crude culture, the kit comprising:
- a zero-mode waveguide (ZMW) array comprising a plurality of ZMW nanopores arranged in parallel, each ZMW nanopore being configured to hold a single extracellular vesicle;
- a detection reagent;
- optionally, buffers and receptacles;
- and
- optionally, one or more of packaging, a label, or instructions for use.
27-29. (canceled)
Dependencies are inferred from the claim text.