Method and device for signal collection
AssigneeThermo Fisher Scientific (Shanghai) Instruments Co., Ltd.
InventorsShenghai Wu, Yong Li, Fei Yang, Laigui Qin
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AssigneeThermo Fisher Scientific (Shanghai) Instruments Co., Ltd.
InventorsShenghai Wu, Yong Li, Fei Yang, Laigui Qin
Estimating the term…
Methods for performing signal collection on fluorescence emitted by an object to be subjected to detection in a sample comprise: performing photoelectric detection on fluorescence emitted by the object to generate an electrical signal; integrating the electrical signal; collecting the integrated electrical signal; and in response to a trigger indication, releasing, within a time period, more than 90% of charges accumulated in a charge integrator that integrates the electrical signal, wherein the time period is less than 15% of a full width at half maximum of a time-domain waveform corresponding to fluorescence intensity. By releasing most of the accumulated charges in a timely manner within a short time during the process of integrating an electrical signal generated during fluorescence detection, the problem of the dynamic range of a fluorescence detector being insufficient is effectively solved, such that the dynamic range can be expanded.
A signal acquisition method for fluorescence emitted by an object to be detected in a sample, comprising:
A signal acquisition device, comprising:
Dependencies are inferred from the claim text.