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OwnerDimitrios STAMOU, Gabriele KOCKELKOREN +4inventors
InventorsDimitrios STAMOU, Gabriele KOCKELKOREN, Line LAURITSEN, Christopher SHUTTLE, Artu BREUER, Eieftheria KAZEPIDOU
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The present invention suggests a method and a system for characterizing a membrane bound molecule. The method comprises determining a plurality of spatially resolved membrane properties of a biological membrane, determining, for a plurality of locations, a spatially resolved membrane molecule signal related to the membrane bound molecule at the plurality of locations on the membrane and grouping the spatially resolved membrane molecule signals to classes of at least one of the membrane properties or state classes of the membrane bound molecules. The method further comprises identifying the distribution of conformational states of the membrane bound molecule based on the distribution of the classes.
A method for characterizing a distribution of states of a membrane bound molecule, the method comprising:
The method of any one of the preceding claims, further comprising obtaining patterns of the distribution of conformational states of the membrane bound molecules.
The method of any one of the preceding claims, further comprising inducing a conformational change to the membrane bound molecule.
The method of any one of the preceding claims, wherein the determining the spatially resolved membrane molecule signal comprises detecting a signal of at least one of a tag attached to the membrane molecule, an antibody to the membrane bound molecule, a conformational biosensor, a ligand to the membrane bound molecule.
The method of any one of the preceding claims, wherein the determining the spatially resolved membrane molecule signal comprises determining at least one of a ratio of signals of an antibody to membrane bound molecule, ligand to antibody, ligand to membrane bound molecule, a conformational biosensor to membrane bound molecule, antibody to conformational biosensor, and ligand to conformational biosensor.
The method of any one of the preceding claims, wherein the determining the spatially resolved membrane molecule signal comprises determining a signal of the membrane bound molecule and further comprising determining an additional spatially resolved molecules signal relating to at least one of a ligand, an antibody and a conformational biosensor.
The method of any one of the preceding claims, wherein the determining the spatially resolved membrane molecule signal comprises determining, for the plurality of locations, a state specific spatially resolved membrane molecule signal relating to a conformational state of the membrane bound molecule.
The method of any one of the preceding claims, wherein the biological membrane is a plasma membrane of a biological cell.
The method of any one of the preceding claims, wherein the membrane molecule is a membrane bound receptor protein.
The method of any one of the preceding claims, wherein the plurality of spatially resolved membrane properties are topography parameters of the membrane.
A method for characterizing a state of a membrane bound molecule, the method comprising:
A method for characterizing a membrane bound molecule, the method comprising:
An apparatus for characterizing a membrane bound molecule, the apparatus comprising a fluorescence imaging device for imaging fluorescence in and at cell membranes and a data evaluation, the data evaluation implementing the methods of any of the preceding claims.
A data storage comprising data representative for a state specific membrane molecule pattern in a membrane property spaces and representative for the conformational state of the membrane molecule.
Dependencies are inferred from the claim text.