KIT FOR GENOTYPING OR DETECTION OF HPVs, METHOD AND SYSTEM USING SAID KIT

A kit for detecting and simultaneously genotyping a plurality of Human Papillomavirus (HPV) genotypes in a biological sample, comprising:

• a plurality of physically discrete reaction pools configured to collectively detect and differentiate said plurality of HPV genotypes;
• wherein each reaction pool comprises a formulated, non-overlapping subset of viral gene loci-specific oligonucleotide triplets, each triplet comprising a forward primer, a reverse primer, and a sequence-specific dual-labeled fluorescent DNA probe;
• wherein the oligonucleotide triplets within the kit are configured to specifically hybridize to and amplify a synergistic combination of viral genetic targets, said targets comprising a highly conserved L1 structural gene and at least one early viral gene selected from the group consisting of an E4 gene and an E6 gene, thereby mitigating false-negative detection associated with viral episomal integration;
• and wherein at least one of said reaction pools further comprises an internal control primer-probe set targeting an endogenous human housekeeping gene to verify sample adequacy and the absence of amplification inhibitors.