Method for assessing impact of transgenic plants on agricultural biodiversity using environmental dna metabarcoding sequencing

Abstract

The present disclosure relates to a method for assessing an impact of a transgenic plant on agricultural biodiversity using environmental DNA (eDNA) metabarcoding sequencing. The method includes the following steps: collecting rhizosphere soil samples and phyllosphere surface attachment samples of a transgenic crop during the same period, and extracting deoxyribonucleic acid (DNA) from the samples; using the extracted DNA as a template, performing polymerase chain reaction (PCR) amplification with amplification primers; and purifying PCR amplification products, subjecting the purified PCR amplification products to high-throughput sequencing (HTS) to obtain sequences, comparing the obtained operational taxonomic unit (OTU) sequences with the nucleotide (nt) public database on the website of the National Center for Biotechnology Information (NCBI) to identify arthropod species in the samples and calculate biodiversity-related indices, thereby assessing the impact of the transgenic crop on agricultural biodiversity.

Dependencies are inferred from the claim text.