Compositions and methods for detecting group b streptococcus nucleic acid

A composition for determining the presence or absence of Group B Streptococcus (GBS) in a sample, said composition comprising:

• at least one first amplification oligomer combination and at least one second amplification oligomer combination, wherein
• (I) the first amplification oligomer combination comprises first and second SIP-specific amplification oligomers capable of amplifying a target region of a GBS SIP target nucleic acid, wherein the first and second SIP-specific amplification oligomers comprise, respectively, first (A) and second (B) SIP-specific target-hybridizing sequences selected from the group consisting of:
• (a) (A) SEQ ID NO:3, or an RNA equivalent or DNA/RNA chimeric thereof, and (B) SEQ ID NO:4, or an RNA equivalent or DNA/RNA chimeric thereof, and
• (b) (A) SEQ ID NO:7, or an RNA equivalent or DNA/RNA chimeric thereof, and (B) SEQ ID NO:8, or an RNA equivalent or DNA/RNA chimeric thereof,
• and
• (II) the second amplification oligomer combination comprises first and second CFB-specific amplification oligomers capable of amplifying a target region of a GBS CFB target nucleic acid, wherein the first and second CFB-specific amplification oligomers comprise, respectively, first (A′) and second (B′) CFB-specific target-hybridizing sequences selected from the group consisting of:
• (a) (A′) SEQ ID NO:16, or an RNA equivalent or DNA/RNA chimeric thereof, and (B′) SEQ ID NO:17, or an RNA equivalent or DNA/RNA chimeric thereof, and
• (b) (A′) SEQ ID NO:20, or an RNA equivalent or DNA/RNA chimeric thereof, and (B′) SEQ ID NO:21, or an RNA equivalent or DNA/RNA chimeric thereof.