Back to results
US 2026/0275375 A1Published application
Synthetic circuits and uses thereof
AssigneeStrand Therapeutics Inc.
InventorsTasuku KITADA, Jaspreet KHURANA, Wen Allen TSENG, Justin LETENDRE, Rachel EICHMAN, Sarah BENING, Aidan SIMPSON, Dahyana ARIAS ESCAYOLA
Life of this patent
Estimating the term…
Abstract
The present disclosure relates to a synthetic circuit that can be used to selectively express a payload in a target cell. In some aspects, a synthetic circuit comprises a payload sequence comprising a sensor and a regulator sequence comprising a sensor. Also provided herein are methods of using such synthetic circuits to treat a wide range of disease or disorders.
Claims163
A synthetic circuit comprising:
- (a) a first nucleotide sequence encoding a payload (payload sequence) and
- (b) a second nucleotide sequence encoding a regulator (regulator sequence),
- wherein the payload sequence comprises a sensor that is capable of specifically recognizing the regulator (type P sensor),
- wherein the regulator sequence comprises a sensor that is capable of specifically recognizing a marker (type R sensor), and
- wherein the regulator and the marker recognized by the type R sensor (type R marker) are not the same.
2 more elements
A synthetic circuit comprising:
- (a) a first nucleotide sequence encoding a payload (payload sequence) and
- (b) a second nucleotide sequence encoding a regulator (regulator sequence),
- wherein the payload sequence comprises a first sensor that is capable of specifically recognizing the regulator (first type P sensor) and a second sensor that is capable of specifically recognizing a marker (second type P sensor),
- wherein the regulator sequence comprises a sensor that is capable of specifically recognizing a marker (type R sensor), and
- wherein the regulator, the marker recognized by the second type P sensor (second type P marker), and/or the marker recognized by the type R sensor (type R marker) are not the same.
2 more elements
A synthetic circuit comprising:
- (a) a first nucleotide sequence encoding a payload (payload sequence) and
- (b) a second nucleotide sequence encoding a regulator (regulator sequence);
- wherein the payload sequence is a self-replicating RNA and comprises a sensor that is capable of specifically recognizing the regulator (type P sensor);
- wherein the regulator sequence is a circular RNA and comprises a sensor that is capable of specifically recognizing a marker (type R sensor);
- and
- wherein the regulator and the marker recognized by the type R sensor (type R marker) are not the same.
3 more elements
A synthetic circuit comprising:
- (a) a first nucleotide sequence encoding a payload (payload sequence) and (b) a second nucleotide sequence encoding a regulator (regulator sequence);
- wherein the payload sequence is a self-replicating RNA and comprises a first sensor that is capable of specifically recognizing the regulator (first type P sensor) and a second sensor that is capable of specifically recognizing a marker (second type P sensor);
- wherein the regulator sequence is a circular RNA and comprises a sensor that is capable of specifically recognizing a marker (type R sensor);
- and
- wherein the regulator, the marker recognized by the second type P sensor (type P marker), and the marker recognized by the type R sensor (type R marker) are not the same.
2 more elements
A synthetic circuit comprising:
- (a) a first nucleotide sequence encoding a payload (payload sequence) and
- (b) a second nucleotide sequence encoding a regulator (regulator sequence);
- wherein the payload sequence is a self-replicating RNA comprises a sensor that is capable of specifically recognizing the regulator (type P sensor);
- wherein the regulator sequence is a non-replicating linear RNA and comprises a sensor that is capable of specifically recognizing a marker (type R sensor);
- and
- wherein the regulator and the marker recognized by the type R sensor (type R marker) are not the same.
3 more elements
A synthetic circuit comprising:
- (a) a first nucleotide sequence encoding a payload (payload sequence) and (b) a second nucleotide sequence encoding a regulator (regulator sequence);
- wherein the payload sequence is a self-replicating RNA and comprises a first sensor that is capable of specifically recognizing the regulator (first type P sensor) and a second sensor that is capable of specifically recognizing a marker (second type P sensor);
- wherein the regulator sequence is a non-replicating linear RNA and comprises a sensor that is capable of specifically recognizing a marker (type R sensor);
- and
- wherein the regulator, the marker recognized by the second type P sensor (type P marker), and the marker recognized by the type R sensor (type R marker) are not the same.
2 more elements
A synthetic circuit comprising:
- (a) a first nucleotide sequence encoding a payload (payload sequence) and
- (b) a second nucleotide sequence encoding a regulator (regulator sequence);
- wherein the payload sequence is a circular RNA and comprises a sensor that is capable of specifically recognizing the regulator (type P sensor);
- wherein the regulator sequence is a circular RNA and comprises a sensor that is capable of specifically recognizing a marker (type R sensor);
- and
- wherein the regulator and the marker recognized by the type R sensor (type R marker) are not the same.
3 more elements
A synthetic circuit comprising:
- (a) a first nucleotide sequence encoding a payload (payload sequence) and (b) a second nucleotide sequence encoding a regulator (regulator sequence);
- wherein the payload sequence is a circular RNA and comprises a first sensor that is capable of specifically recognizing the regulator (first type P sensor) and a second sensor that is capable of specifically recognizing a marker (second type P sensor);
- wherein the regulator sequence is a circular RNA and comprises a sensor that is capable of specifically recognizing a marker (type R sensor);
- and
- wherein the regulator, the marker recognized by the second type P sensor (type P marker), and the marker recognized by the type R sensor (type R marker) are not the same.
2 more elements
A synthetic circuit comprising:
- (a) a first nucleotide sequence encoding a payload (payload sequence) and
- (b) a second nucleotide sequence encoding a regulator (regulator sequence);
- wherein the payload sequence is a circular RNA and comprises a sensor that is capable of specifically recognizing the regulator (type P sensor);
- wherein the regulator sequence is a non-replicating linear RNA and comprises a sensor that is capable of specifically recognizing a marker (type R sensor);
- and
- wherein the regulator and the marker recognized by the type R sensor (type R marker) are not the same.
3 more elements
A synthetic circuit comprising:
- (a) a first nucleotide sequence encoding a payload (payload sequence) and (b) a second nucleotide sequence encoding a regulator (regulator sequence);
- wherein the payload sequence is a circular RNA and comprises a first sensor that is capable of specifically recognizing the regulator (first type P sensor) and a second sensor that is capable of specifically recognizing a marker (second type P sensor);
- wherein the regulator sequence is a non-replicating linear RNA and comprises a sensor that is capable of specifically recognizing a marker (type R sensor);
- and
- wherein the regulator, the marker recognized by the second type P sensor (type P marker), and the marker recognized by the type R sensor (type R marker) are not the same.
2 more elements
A synthetic circuit comprising:
- (a) a first nucleotide sequence encoding a payload (payload sequence) and
- (b) a second nucleotide sequence encoding a regulator (regulator sequence),
- wherein, when the payload sequence and the regulator sequence are present in a target cell, the payload is expressed in the target cell for a first expression and the regulator is expressed in the target cell for a second expression, and
- wherein the first expression is greater than the second expression.
1 more element
A synthetic circuit comprising:
- (a) a first nucleotide sequence encoding a payload (payload sequence) and
- (b) a second nucleotide sequence encoding a regulator (regulator sequence),
- wherein, when the payload sequence and the regulator sequence are present in a non-target cell, the payload is expressed in the non-target cell for a first expression and the regulator is expressed in the non-target cell for a second expression, and
- wherein the second expression is greater than the first expression.
1 more element
A synthetic circuit comprising:
- (a) a first nucleotide sequence encoding a payload (payload sequence) and
- (b) a second nucleotide sequence encoding a regulator (regulator sequence),
- wherein, when the synthetic circuit is contacted with a population of cells comprising a target cell and a non-target cell, the expression of the payload in the target cell is higher than the corresponding expression in the non-target cell.
Dependencies are inferred from the claim text.