Mutant of type iii pullulan hydrolase with enhanced enzymatic activity and construction method thereof
AssigneeInstitute of Microbiology, Jiangxi Academy of Sciences (Jiangxi Institute of Watershed Ecology)
InventorsJing ZENG, Xuaner LUO, Lin YUAN, Jianjun GUO, Dawei XIONG, Shuaiwen ZHANG, Tong WANG
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Abstract
A mutant of Type III pullulan hydrolase with enhanced enzymatic activity and a construction method thereof are provided. The amino acid sequence of the type III pullulan hydrolase mutant is shown in SEQ ID NO. 3. On the basis of type III pullulan hydrolase TK-PUL from Thermococcus kodakarensis, the disclosure develops a mutant of type III pullulan hydrolase with enhanced enzymatic activity through site-directed mutagenesis, shows a 1.83-fold increase in specific enzymatic activity against cassava starch.
Claims3
A mutant of Type III pullulan hydrolase, wherein an amino acid sequence is shown in SEQ ID NO. 3.
Dependencies are inferred from the claim text.