Compositions and methods for preservation of isolated mitochondria
AssigneeThe Sallie A. Burdine Breast Foundation
InventorsXianpeng Jiang, Catherine Baucom, Brent M. Segal
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Abstract
Described herein are compositions and methods for the preservation of the membrane potential of isolated mitochondria, such mitochondria to be used in mitochondrial organelle transplantation methods and compositions. For example, the present disclosure provides for methods and compositions for preservation of isolated mitochondria using extracellular vesicles (EVs).
Claims57
A composition, the composition comprising:
- isolated mitochondria;
- and
- extracellular vesicles (EVs).
A method comprising using a composition comprising isolated mitochondria and extracellular vesicles to improve preservation of mitochondrial membrane potential (MMP) of the isolated mitochondria, wherein the isolated mitochondria are used in any of the mitochondrial organelle transplantation methods and/or compositions and/or kits described in the above-referenced International (PCT) Patent Application No. PCT/US2020/047359 or U.S. Provisional Patent Application No. 63/405,336, both incorporated herein by reference.
A method for transplantation of mitochondria in a human subject, said method comprising:
- storing the mitochondria isolated from a donor in a storage composition comprising extracellular vesicles (EVs);
- and
- after the storing step, administering to said subject a mitochondrial composition comprising said stored mitochondria.
A kit comprising a mitochondrial composition in a unit dosage effective to treat a neurodegenerative disease or other condition associated with mitochondrial dysfunction in a subject, said mitochondrial composition comprising:
- isolated mitochondria;
- and
- extracellular vesicles (EVs).
A method of storing a composition comprising isolated mitochondria and extracellular vesicles to improve preservation of MMP of the isolated mitochondria and/or to improve preservation/retention of mitochondrial adenosine triphosphate (ATP) content, the method comprising storing said composition at a reduced temperature.
Dependencies are inferred from the claim text.