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AssigneesNovozymes A/S, Novo Nordisk Pharmatech A/S
InventorsTine HOFF, Lars Kobberoee SKOV, Karina Sandgaard KRISTENSEN, Anna Verena REISER, Casper WILKENS
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The present invention relates to use of a polypeptide having a sequence identity of at least 70% to SEQ ID NO:1, a mature polypeptide of SEQ ID NO:1, SEQ ID NO:2, or SEQ ID NO:3, wherein the polypeptide has chymopapain activity, in a cell detachment process. The present invention also relates to a method of cell detachment, comprising contacting a cell with a polypeptide having a sequence identity of at least 70% to SEQ ID NO:1, a mature polypeptide of SEQ ID NO:1, SEQ ID NO:2, or SEQ ID NO:3, wherein the polypeptide has chymopapain activity, and wherein the cell is attached to a surface and/or to another cell. The present invention further relates to compositions suitable for cell detachment comprising said polypeptide, polynucleotides encoding said polypeptide, recombinant host cells transformed with said polypeptide, and methods of producing said polypeptide.
1-3. (canceled)
A method of cell detachment, comprising contacting a cell with a polypeptide having a sequence identity of at least 70% to SEQ ID NO:1, a mature polypeptide of SEQ ID NO:1, SEQ ID NO:2, or SEQ ID NO:3, wherein the polypeptide has chymopapain activity, wherein the cell is attached to a surface and/or to another cell.
A composition suitable for cell detachment comprising a polypeptide having a sequence identity of at least 70% to SEQ ID NO:1, a mature polypeptide of SEQ ID NO:1, SEQ ID NO:2, or SEQ ID NO:3, wherein the polypeptide has chymopapain activity.
A polynucleotide encoding a polypeptide having a sequence identity of at least 70% to SEQ ID NO:1, a mature polypeptide of SEQ ID NO:1, SEQ ID NO:2, or SEQ ID NO:3, wherein the polypeptide has chymopapain activity.
The recombinant host cell according to claim 12, which is an Aspergillus cell.
Dependencies are inferred from the claim text.