Methods for generating artificial thymus, products and uses thereof
OwnerFrank Jakob Theodor STAAL, Aline ZBINDEN +3inventors
InventorsFrank Jakob Theodor STAAL, Aline ZBINDEN, Henricus Johannes Hermanus Maria CLASSEN, Arno BISSCHOP, Max Jurie RENES
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Abstract
The invention provides a method for producing a three dimensional thymic like structure, the method comprising: a) providing pluripotent cells from a recipient with a nucleic acid molecule comprising a FOXN1 sequence, and b) inducing said pluripotent cell to differentiate into thymic epithelium in a scaffold suitable for transplantation, as well as related methods, compositions or uses.
Claims49
A method for producing a three dimensional thymic like structure, the method comprising:
- a) providing pluripotent cell(s) from a recipient with a nucleic acid molecule comprising a FOXN1 sequence, and b) inducing said pluripotent cell(s) to differentiate into thymic epithelium in a scaffold suitable for transplantation.
A method for producing a three dimensional thymic like structure, the method comprising:
- a) providing pluripotent cell(s) from a recipient, and b) inducing said pluripotent cell(s) to differentiate into thymic epithelium in a scaffold suitable for transplantation, wherein a nucleic acid molecule comprising a FOXN1 sequence is provided to the pluripotent cell(s) from a recipient of a) or to cells of a differentiation stage in b) selected from definitive endoderm cells, anterior foregut endoderm cells, and pharyngeal pouch endoderm cells.
A nucleic acid molecule comprising a FOXN1 sequence, wherein the nucleic acid further comprises nucleic acid sequences for introducing said FOXN1 sequence in a pluripotent cell.
A thymus epithelial progenitor cell (TEPC) comprising a FOXN1 encoding sequence integrated in its DNA in a safe harbour locus.
A TEPC comprising a FOXN1 encoding sequence integrated in its DNA.
A thymus spheroid or organoid according to any one of claims 27-34, wherein the thymus spheroid or organoid has a medulla-like and/or cortex-like three-dimensional structure.
Dependencies are inferred from the claim text.