Cells being highly enriched in sasp factors, and composition comprising said cells
AssigneeEvomedis GmbH
InventorMartin Funk
Life of this patent
Estimating the term…
Abstract
The present invention refers to a cell having the capacity of producing at least one SASP factor, obtainable or obtained by treating the cell with an amount of an exogenous secretion inhibitor that is sufficient to inhibit the secretion of the at least one SASP-factor. The present invention further refers to a cell that is intracellularly enriched in at least one SASP factor selected from a defined group of SASP factors, to a composition comprising said cells, as well as to this cell and composition for use in a therapeutic and/or prophylactic treatment, and also for use in tissue regeneration. The present invention also refers to a method for producing a cell that is intracellularly enriched in at least one endogenously produced SASP factor.
Claims25
A cell having the capacity of producing at least one SASP factor, obtainable or obtained by treating the cell with an amount of an exogenous secretion inhibitor that is sufficient to reduce the secretion of the at least one SASP-factor.
A cell being intracellularly enriched in at least one SASP-factor selected from the group consisting of IL-1 alpha, IL-6, IL8, TGF-alpha, GRO alpha, VEGF, EGF, FGF acid, MMP7, and TIMP1, wherein the intracellular concentration, calculated as pg/mg protein extract, of said one or more SASP factor, if present, is defined as follows:
- intracellular
- (pg/mg protein
- extract)
- IL-1 alpha
- more than 80
- IL-6
- more than 2
- TGF- alpha
- more than 20
- IL8
- more than 20
- GRO alpha
- more than 20
- VEGF
- more than 20
- EGF
- more than 10
- FGF acidic
- more than 100
- MMP7
- more than 200
- TIMP1
- TIMP1
- more than 200
12-29. (canceled)
A method for producing a cell that is intracellularly enriched in at least one endogenously produced SASP factor, comprising the following steps:
- (a) providing a cell that is capable of producing one or more SASP factors;
- (b) incubating the cell of (i) under suitable conditions with an exogenous secretion inhibitor in an amount that is sufficient to reduce the secretion of the at least one SASP-factor;
- (c) obtaining the cell of (ii) that is intracellularly enriched in at least one endogenously produced SASP factor.
A method for producing a cell that is intracellularly enriched in at least one endogenously produced SASP factor, comprising the following steps:
- (a) providing a cell that is capable of producing one or more SASP factors;
- (b) incubating the cell of (i) under suitable conditions with an exogenous secretion inhibitor in an amount that is sufficient to reduce the secretion of the at least one SASP-factor;
- (c) obtaining the cell of (ii) that is intracellularly enriched in at least one endogenously produced SASP factor.
Dependencies are inferred from the claim text.