Compositions and methods for differentiating rpe cells
AssigneeStemcell Technologies Canada Inc.
InventorsIshpreet DHILLON, Valentina MARCHETTI, Li Jing SHEAVES
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Abstract
The present disclosure relates to applications of culturing retinal pigment epithelium (RPE) cells. The method comprises a) providing a population of PSC; b) exposing the population of PSC to a first culture environment comprising an extracellular matrix protein and an inhibitor of BMP signaling; c) culturing the population of PSC in the first culture environment for between about 0 to 60 hours; and d) deriving the population of cells fated for the retinal linage.
Claims27
A method of differentiating a population of cells fated for the retinal lineage, the method comprising:
- a) providing a population of pluripotent stem cells (PSC);
- b) exposing the population of PSC to a first culture environment comprising an extracellular matrix protein and an inhibitor of bone morphogenetic protein (BMP) signaling in a first cell culture medium;
- c) culturing the population of PSC in the first culture environment for between about 0 to 60 hours;
- and
- d) deriving the population of cells fated for the retinal linage.
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Dependencies are inferred from the claim text.