US 2026/0275248 A1Published application

Glycosyl hydrolases

AssigneeNovozymes A/S

InventorsChristian Berg OEHLENSCHLAEGER, Rebecca Munk VEJBORG, Dorotea Raventos SEGURA, Jesper SOLOMON

Life of this patent

Estimating the term…

Abstract

The invention relates to polypeptides having hydrolytic activity and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.

Claims6

Dependent claims

A Glyco_hydro_114 glycosyl hydrolase comprising one, two or all three of the motifs GX[FY][LYF]D (SEQ ID NO:

34), AYX[SET]XX[EAS] (SEQ ID NO:
35) and GXXGX[FY][LYFI]D (SEQ ID NO:
97), wherein the Glyco_hydro_114 glycosyl hydrolase has hydrolytic activity, and wherein the Glyco_hydro_114 glycosyl hydrolase comprises or consists of a polypeptide selected from the group consisting of:
(a) a polypeptide having at least 90% sequence identity to the polypeptide of SEQ ID NO:
3;
(b) a polypeptide having at least 65% sequence identity to the polypeptide of SEQ ID NO:
6;
(c) a polypeptide having at least 80% sequence identity to the polypeptide of SEQ ID NO:
9;
(d) a polypeptide having at least 99% sequence identity to the polypeptide of SEQ ID NO:
12;
(e) a polypeptide having at least 95% sequence identity to the polypeptide of SEQ ID NO:
15;
(f) a polypeptide having at least 95% sequence identity to the polypeptide of SEQ ID NO:
18;
(g) a polypeptide having at least 60% sequence identity to the polypeptide of SEQ ID NO:
21;
(h) a polypeptide having at least 80% sequence identity to the polypeptide of SEQ ID NO:
24;
(i) a polypeptide having at least 70% sequence identity to the polypeptide of SEQ ID NO:
27;
(j) a polypeptide having at least 65% sequence identity to the polypeptide of SEQ ID NO:
30;
(k) a polypeptide having at least 70% sequence identity to the polypeptide of SEQ ID NO:
33;
(l) a polypeptide having 100% sequence identity to the polypeptide of SEQ ID NO:
40;
(m) a polypeptide having at least 95% sequence identity to the polypeptide of SEQ ID NO:
43;
(n) a polypeptide having 100% sequence identity to the polypeptide of SEQ ID NO:
46;
(o) a polypeptide having at least 95% sequence identity to the polypeptide of SEQ ID NO:
49;
(p) a polypeptide having at least 95% sequence identity to the polypeptide of SEQ ID NO:
52;
(q) a polypeptide having at least 95% sequence identity to the polypeptide of SEQ ID NO:
55;
(r) a polypeptide having at least 75% sequence identity to the polypeptide of SEQ ID NO:
58;
(s) a polypeptide having at least 95% sequence identity to the polypeptide of SEQ ID NO:
61;
(t) a polypeptide having at least 90% sequence identity to the polypeptide of SEQ ID NO:
64;
(u) a polypeptide having at least 65% sequence identity to the polypeptide of SEQ ID NO:
67;
(v) a polypeptide having at least 96% sequence identity to the polypeptide of SEQ ID NO:
73;
(w) a polypeptide having at least 96% sequence identity to the polypeptide of SEQ ID NO:
76;
(x) a polypeptide having at least 60% sequence identity to the polypeptide of SEQ ID NO:
79;
(y) a polypeptide having at least 95% sequence identity to the polypeptide of SEQ ID NO:
82;
(z) a polypeptide having at least 50% sequence identity to the polypeptide of SEQ ID NO:
85;
(aa) a polypeptide having at least 50% sequence identity to the polypeptide of SEQ ID NO:
88;
(bb) a polypeptide having at least 65% sequence identity to the polypeptide of SEQ ID NO:
91;
(cc) a polypeptide having at least 80% sequence identity to the polypeptide of SEQ ID NO:
94;
(dd) a polypeptide having at least 70% sequence identity to the polypeptide of SEQ ID NO:
95;
and
(ee) a polypeptide having at least 95% sequence identity to the polypeptide of SEQ ID NO:
96.

63 more elements

Dependencies are inferred from the claim text.

Facts

Application: 19/407,182Filed 3 Dec 2025
Priority: EP 17164859.54 Apr 2017
Published: 17 Sep 2026
Term ends: Not estimatedThe source records don’t support an estimate.

Classification7 codes

C11D 3/38636
Preparations containing enzymes , e.g. protease or amylase: containing enzymes other than protease, amylase, lipase, cellulase, oxidase or reductase
C11D 3/38672
Preparations containing enzymes , e.g. protease or amylase: granulated or coated enzymes
C11D 17/0039
Detergent materials or soaps characterised by their shape or physical properties: coated compositions or coated components in the compositions,…
C12N 9/2402
Acting on glycosyl compounds: hydrolysing O- and S- glycosyl compounds

Family

Application16/499,877Division · filed 4 Apr 2018
Application17/717,601Division · filed 11 Apr 2022
Application19/407,182Division
US 11,339,355 B2Related patent
US 12,516,273 B2Related patent
US 2020/0109353 A1Published application
US 2022/0340842 A1Published application
US 2026/0275248 A1This patent

From the US filing history. Foreign family members aren’t listed.

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Abstract

A Glyco_hydro_114 glycosyl hydrolase comprising one, two or all three of the motifs GX[FY][LYF]D (SEQ ID NO:

34), AYX[SET]XX[EAS] (SEQ ID NO:
35) and GXXGX[FY][LYFI]D (SEQ ID NO:
97), wherein the Glyco_hydro_114 glycosyl hydrolase has hydrolytic activity, and wherein the Glyco_hydro_114 glycosyl hydrolase comprises or consists of a polypeptide selected from the group consisting of:
(a) a polypeptide having at least 90% sequence identity to the polypeptide of SEQ ID NO:
3;
(b) a polypeptide having at least 65% sequence identity to the polypeptide of SEQ ID NO:
6;
(c) a polypeptide having at least 80% sequence identity to the polypeptide of SEQ ID NO:
9;
(d) a polypeptide having at least 99% sequence identity to the polypeptide of SEQ ID NO:
12;
(e) a polypeptide having at least 95% sequence identity to the polypeptide of SEQ ID NO:
15;
(f) a polypeptide having at least 95% sequence identity to the polypeptide of SEQ ID NO:
18;
(g) a polypeptide having at least 60% sequence identity to the polypeptide of SEQ ID NO:
21;
(h) a polypeptide having at least 80% sequence identity to the polypeptide of SEQ ID NO:
24;
(i) a polypeptide having at least 70% sequence identity to the polypeptide of SEQ ID NO:
27;
(j) a polypeptide having at least 65% sequence identity to the polypeptide of SEQ ID NO:
30;
(k) a polypeptide having at least 70% sequence identity to the polypeptide of SEQ ID NO:
33;
(l) a polypeptide having 100% sequence identity to the polypeptide of SEQ ID NO:
40;
(m) a polypeptide having at least 95% sequence identity to the polypeptide of SEQ ID NO:
43;
(n) a polypeptide having 100% sequence identity to the polypeptide of SEQ ID NO:
46;
(o) a polypeptide having at least 95% sequence identity to the polypeptide of SEQ ID NO:
49;
(p) a polypeptide having at least 95% sequence identity to the polypeptide of SEQ ID NO:
52;
(q) a polypeptide having at least 95% sequence identity to the polypeptide of SEQ ID NO:
55;
(r) a polypeptide having at least 75% sequence identity to the polypeptide of SEQ ID NO:
58;
(s) a polypeptide having at least 95% sequence identity to the polypeptide of SEQ ID NO:
61;
(t) a polypeptide having at least 90% sequence identity to the polypeptide of SEQ ID NO:
64;
(u) a polypeptide having at least 65% sequence identity to the polypeptide of SEQ ID NO:
67;
(v) a polypeptide having at least 96% sequence identity to the polypeptide of SEQ ID NO:
73;
(w) a polypeptide having at least 96% sequence identity to the polypeptide of SEQ ID NO:
76;
(x) a polypeptide having at least 60% sequence identity to the polypeptide of SEQ ID NO:
79;
(y) a polypeptide having at least 95% sequence identity to the polypeptide of SEQ ID NO:
82;
(z) a polypeptide having at least 50% sequence identity to the polypeptide of SEQ ID NO:
85;
(aa) a polypeptide having at least 50% sequence identity to the polypeptide of SEQ ID NO:
88;
(bb) a polypeptide having at least 65% sequence identity to the polypeptide of SEQ ID NO:
91;
(cc) a polypeptide having at least 80% sequence identity to the polypeptide of SEQ ID NO:
94;
(dd) a polypeptide having at least 70% sequence identity to the polypeptide of SEQ ID NO:
95;
and
(ee) a polypeptide having at least 95% sequence identity to the polypeptide of SEQ ID NO:
96.

63 more elements

Facts

Application

19/407,182Filed 3 Dec 2025

Priority

EP 17164859.54 Apr 2017

Published

17 Sep 2026

Term ends

Not estimatedThe source records don’t support an estimate.

Classification7 codes

C11D 3/38636
Preparations containing enzymes , e.g. protease or amylase: containing enzymes other than protease, amylase, lipase, cellulase, oxidase or reductase
C11D 3/38672
Preparations containing enzymes , e.g. protease or amylase: granulated or coated enzymes
C11D 17/0039
Detergent materials or soaps characterised by their shape or physical properties: coated compositions or coated components in the compositions,…
C12N 9/2402
Acting on glycosyl compounds: hydrolysing O- and S- glycosyl compounds

Family

Application16/499,877Division · filed 4 Apr 2018

Application17/717,601Division · filed 11 Apr 2022

Application19/407,182Division

US 11,339,355 B2Related patent

US 12,516,273 B2Related patent

US 2020/0109353 A1Published application

US 2022/0340842 A1Published application

US 2026/0275248 A1This patent

From the US filing history. Foreign family members aren’t listed.