Woolly mammoth specific gene variants and compositions comprising same
AssigneeColossal Biosciences Inc.
InventorsEriona HYSOLLI, Austin BOW, Michael ABRAMS, Ben LAMM, Devon FITZPATRICK
Life of this patent
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Abstract
Provided herein are isolated nucleic acids, vectors, recombinant cells, and transgenic animals capable of expressing at least one or more woolly mammoth genes. Also provided are recombinant cells and transgenic animals that comprise one or more woolly mammoth specific deletions of regulatory regions upstream of identified transcription start sites.
Claims27
An isolated nucleic acid sequence comprising a nucleotide sequence having at least 80%, at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, or at least 99% identity to at least one of G protein-coupled receptor 98 (GPR98) (SEQ ID NO:1), microtubule actin crosslinking factor 1 (MACF1) (SEQ ID NO:2), adenosine deaminase RNA specific B2 (ADARB2) (SEQ ID NO:3), centrosomal protein 290 (CEP290) (SEQ ID NO:4), keratin 4 (KRT4) (SEQ ID NO:5), LOC126076011 (SEQ ID NO:6), NCK associated protein 5 (NCKAP5) (SEQ ID NO:7), laminin subunit beta 4 (LAMB4) (SEQ ID NO:8), Niemann-Pick C1-like 1 (NPC1L1) (SEQ ID NO:9), adhesion G protein-coupled receptor D2 (ADGRD2) (SEQ ID NO:10), ninjurin 1 (NINJ1) (SEQ ID NO:11), AHNAK nucleoprotein 2 (AHNAK2) (SEQ ID NO:12), cation channel sperm associated auxiliary subunit beta (CATSPERB) (SEQ ID NO:13), pecanex-like 4 (PCNXL4) (SEQ ID NO:14), spectrin repeat containing nuclear envelope protein 2 (SYNE2) (SEQ ID NO:15), NLR family pyrin domain containing 12 (NLRP12) (SEQ ID NO:16), LOC126086768 (SEQ ID NO:17), WD repeat domain 90 (WDR90) (SEQ ID NO:18), breast cancer 2 (BRCA2) (SEQ ID NO:19), protein kinase, DNA-activated catalytic subunit (PRKDC) (SEQ ID NO:20), vacuolar protein sorting 13 homolog B (VPS13B) (SEQ ID NO:21), prosaposin (PSAP) (SEQ ID NO:22), SEC31 homolog B (SEC31B) (SEQ ID NO:23), keratin 28 (KRT28) (SEQ ID NO:24), keratin 35 (KRT35) (SEQ ID NO:25), keratin 40 (KRT40) (SEQ ID NO:26), myosin heavy chain 4 (MYH4) (SEQ ID NO:27), phosphoinositide interacting regulator of TRP (PIRT) (SEQ ID NO:28), polycystin 1 like 2 (PKD1L2) (SEQ ID NO:29), retinitis pigmentosa 1-like protein (RP1L1) (SEQ ID NO:30), chromosome X open reading frame 58 (CXorf58) (SEQ ID NO:31), LOC126068772 (SEQ ID NO:32), LOC126069872 (SEQ ID NO:33), thyroid hormone receptor associated protein 3 (THRAP3) (SEQ ID NO:34), centromere protein C 1 (CENPC1) (SEQ ID NO:35), dentinogenesis and dentin sialophosphoprotein (DSPP) (SEQ ID NO:36), fibroblast growth factor 5 (FGF5) (SEQ ID NO:37), cation channel sperm associated auxiliary subunit gamma (CATSPERG) (SEQ ID NO:38), myosin heavy chain 1 (MYH1) (SEQ ID NO:39), myosin heavy chain 13 (MYH13) (SEQ ID NO:40), ATR interacting protein (ATRIP) (SEQ ID NO:41), and transglutaminase 3 (TGM3) (SEQ ID NO:42).
A recombinant host cell comprising a nucleotide sequence having at least 80%, at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, or at least 99% identity to at least one of GPR98 (SEQ ID NO:1), MACF1 (SEQ ID NO:2), ADARB2 (SEQ ID NO:3), CEP290 (SEQ ID NO:4), KRT4 (SEQ ID NO:5), LOC126076011 (SEQ ID NO:6), NCKAP5 (SEQ ID NO:7), LAMB4 (SEQ ID NO:8), NPC1L1 (SEQ ID NO:9), ADGRD2 (SEQ ID NO:10), NINJ1 (SEQ ID NO:11), AHNAK2 (SEQ ID NO:12), CATSPERB (SEQ ID NO:13), PCNXL4 (SEQ ID NO:14), SYNE2 (SEQ ID NO:15), NLRP12 (SEQ ID NO:16), LOC126086768 (SEQ ID NO:17), WDR90 (SEQ ID NO:18), BRCA2 (SEQ ID NO:19), PRKDC (SEQ ID NO:20), VPS13B (SEQ ID NO:21), PSAP (SEQ ID NO:22), SEC31B (SEQ ID NO:23), KRT28 (SEQ ID NO:24), KRT35 (SEQ ID NO:25), KRT40 (SEQ ID NO:26), MYH4 (SEQ ID NO:27), PIRT (SEQ ID NO:28), PKD1L2 (SEQ ID NO:29), RP1L1 (SEQ ID NO:30), CXorf58 (SEQ ID NO:31), LOC126068772 (SEQ ID NO:32), LOC126069872 (SEQ ID NO:33), THRAP3 (SEQ ID NO:34), CENPC1 (SEQ ID NO:35), DSPP (SEQ ID NO:36), FGF5 (SEQ ID NO:37), CATSPERG (SEQ ID NO:38), MYH1 (SEQ ID NO:39), MYH13 (SEQ ID NO:40), ATRIP (SEQ ID NO:41), and TGM3 (SEQ ID NO:42).
A recombinant host cell comprising a deletion of at least one nucleotide sequence upstream of a transcription start site of LOC126071805, WASH complex subunit 4 (WASHC4), LOC126075532, LOC126075533, alpha-fetoprotein (AFP), LOC126079103, LOC126079327, LOC126079327, LOC126080333, LOC126080367, LOC126080369, LOC126080369, LOC126080733, distal-less homeobox 6 (DLX6), extracellular matrix protein 2 (ECM2), LOC126085059, LOC126085481, LOC126086151, LOC126086152, LOC126086293, LOC126058227, LOC126058390, LOC126058396, forkhead box H1 (FOXH1), LOC126060018, LOC126060570, coiled-coil domain containing 15 (CCDC15), INO80 complex subunit B (INO80B), LOC126062579, LOC126063153, LOC126063990, LOC126063991, LOC126066513, LOC126066877, acyl-CoA wax alcohol acyltransferase 1 (AWAT1), transmembrane protein 187 (TMEM187), and LOC126069912, wherein the deleted nucleic acid sequence upstream of:
- (a) LOC126071805 comprises SEQ ID NO:43,
- (b) WASHC4 comprises SEQ ID NO:44,
- (c) LOC126075532 comprises SEQ ID NO:45,
- (d) LOC126075533 comprises SEQ ID NO:46,
- (e) AFP comprises SEQ ID NO:47,
- (f) LOC126079103 comprises SEQ ID NO:48,
- (g) LOC126079327 comprises SEQ ID NO:49,
- (h) LOC126079327 comprises SEQ ID NO:50,
- (i) LOC126080333 comprises SEQ ID NO:51,
- (j) LOC126080367 comprises SEQ ID NO:52,
- (k) LOC126080369 comprises SEQ ID NO:53,
- (l) LOC126080369 comprises SEQ ID NO:54,
- (m) LOC126080733 comprises SEQ ID NO:55,
- (n) DLX6 comprises SEQ ID NO:56,
- (o) ECM2 comprises SEQ ID NO:57,
- (p) LOC126085059 comprises SEQ ID NO:58,
- (q) LOC126085481 comprises SEQ ID NO:59,
- (r) LOC126086151 comprises SEQ ID NO:60,
- (s) LOC126086152 comprises SEQ ID NO:61,
- (t) LOC126086293 comprises SEQ ID NO:62,
- (u) LOC126058227 comprises SEQ ID NO:63,
- (v) LOC126058390 comprises SEQ ID NO:64,
- (w) LOC126058396 comprises SEQ ID NO:65,
- (x) FOXH1 comprises SEQ ID NO:66,
- (y) LOC126060018 comprises SEQ ID NO:67,
- (z) LOC126060570 comprises SEQ ID NO:68,
- (aa) CCDC15 comprises SEQ ID NO:69,
- (bb) INO80B comprises SEQ ID NO:70,
- (cc) LOC126062579 comprises SEQ ID NO:71,
- (dd) LOC126063153 comprises SEQ ID NO:72,
- (ee) LOC126063990 comprises SEQ ID NO:73,
- (ff) LOC126063991 comprises SEQ ID NO:74,
- (gg) LOC126066513 comprises SEQ ID NO:75,
- (hh) LOC126066877 comprises SEQ ID NO:76,
- (ii) AWAT1 comprises SEQ ID NO:77,
- (jj) TMEM187 comprises SEQ ID NO:78, and
- (kk) LOC126069912 comprises SEQ ID NO:79.
12-14. (canceled)
A transgenic animal comprising at least one woolly mammoth (Mammuthus primigenius) gene variant.
Dependencies are inferred from the claim text.