Antimicrobial ncr2 peptides
AssigneeDonald Danforth Plant Science Center
InventorsDilip M. Shah, Siva L.S. Velivelli
Life of this patent
Estimating the term…
Abstract
Antimicrobial nodule specific cysteine rich (NCR2) peptides and proteins are disclosed along with compositions comprising the NCR2 peptides or NCR2 proteins and transgenic or genetically edited plants or microorganisms that express the NCR2 peptides or proteins to inhibit growth of pathogenic microbes. Such NCR2 peptides, proteins, compositions, plants, and microorganisms can provide for inhibition of fungal and oomycete growth.
Claims24
-3. (canceled)
A recombinant polynucleotide comprising a first polynucleotide encoding a first antimicrobial peptide linked by a spacer peptide to a second antimicrobial peptide, wherein the first and second antimicrobial peptides each comprise one of:
- (i) the amino acid sequence of SEQ ID NO:
- 1, 2, 3, 4, 5, 6, 7, or 40, or a variant thereof wherein one or more of the hydrophobic, basic, and/or acidic amino acid residues are substituted with hydrophobic, basic, and/or acidic amino acid residues, respectively;
- or
- (ii) an amino acid sequence having at least 60%, 70%, 80%, 85%, 90%, 92%, 95%, 97%, 98%, 99%, or 100% sequence identity across the entire length of SEQ ID NO:
- 1, 2, 3, 4, 5, 6, 7, or 40;
- optionally wherein the first or second antimicrobial peptide comprises the amino acid sequence of HKFKGP (SEQ ID NO:
- 34), ISDKE (SEQ ID NO:
- 35), KRRRD (SEQ ID NO:
- 36), or KSRKH (SEQ ID NO:
- 37) at a position located between the first and second cysteine residues of SEQ ID NO:
- 1;
- and/or
- optionally wherein the first or second antimicrobial peptide comprises a C-terminal amino acid sequence of SEQ ID NO:
- 8 or SEQ ID NO:
- 39.
-51. (canceled)
-90. (canceled)
A composition comprising a first antimicrobial peptide linked by a spacer peptide to a second antimicrobial peptide, wherein the first and second antimicrobial peptides each comprise one of:
- (i) the amino acid sequence of SEQ ID NO:
- 1, 2, 3, 4, 5, 6, 7, or 40, or a variant thereof wherein one or more of the hydrophobic, basic, and/or acidic amino acid residues are substituted with hydrophobic, basic, and/or acidic amino acid residues, respectively;
- or
- (ii) an amino acid sequence having at least 60%, 70%, 80%, 85%, 90%, 92%, 95%, 97%, 98%, 99%, or 100% sequence identity across the entire length of SEQ ID NO:
- 1, 2, 3, 4, 5, 6, 7, or 40;
- and an agriculturally, pharmaceutically, or veterinarily acceptable carrier, diluent, or excipient;
- optionally wherein the first and second antimicrobial peptide comprises the amino acid sequence of HKFKGP (SEQ ID NO:
- 34), ISDKE (SEQ ID NO:
- 35), KRRRD (SEQ ID NO:
- 36), or KSRKH (SEQ ID NO:
- 37) at a position located between the first and second cysteine residues of SEQ ID NO:
- 1;
- and/or
- optionally wherein the first or second antimicrobial peptide comprises a C-terminal amino acid sequence of SEQ ID NO:
- 8 or SEQ ID NO:
- 39.
-106. (canceled)
-127. (canceled)
Dependencies are inferred from the claim text.