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US 2026/0273494 A1Published application
Attoliter droplet-based, electrochemical parallel dna synthesizer and dna synthesis method using same
AssigneeKorea University Research and Business Foundation
InventorHong Gu CHUN
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Abstract
The present invention relates to a nanowell array for electrochemical DNA synthesis and a DNA synthesis method using same. By utilizing the nanowell array, the consumption of samples in the electrochemical DNA synthesis process can be reduced, and the diffusion of hydrogen ions can be prevented, thereby reducing synthesis errors.
Claims18
A nanowell array for DNA synthesis, comprising:
- a substrate portion having a plurality of nanowells formed therein, each having a diameter and a depth of less than 1 μm;
- and
- an electrode portion positioned horizontally or vertically with respect to a bottom of the nanowell and including a first electrode and a second electrode.
A nanowell array for DNA synthesis, comprising:
- a substrate portion having a plurality of nanowells formed therein, each having a diameter and a depth of less than 1 μm;
- and
- an electrode portion positioned horizontally or vertically with respect to a bottom of the nanowell and including a first electrode, a second electrode, and a reference electrode.
A DNA synthesis method using the nanowell array of comprising:
- a) a step of supplying a DNA synthesis site selection solution containing a reduction material to the nanowell array;
- b-1) a step of applying, when an electrode portion of the nanowell array includes a first electrode and a second electrode, a first voltage waveform to the first electrode relative to the second electrode to generate hydrogen ions in the DNA synthesis site selection solution;
- or
- b-2) a step of applying, when an electrode portion of the nanowell array includes a first electrode, a second electrode, and a reference electrode, a first voltage waveform to the first electrode relative to the reference electrode to generate hydrogen ions in the DNA synthesis site selection solution;
- and
- c) a step of supplying a solution including a nucleotide to be synthesized.
3 more elements
Dependencies are inferred from the claim text.