Novel materials and methods for refining processes
AssigneeLihme Protein Solutions ApS
InventorsAllan Otto Fog LIHME, Jens SOMMER-KNUDSEN
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Abstract
The present invention relates to a method and apparatus for isolating one or more target biological molecule(s) from a biological composition. In particular, the present invention relates to isolating one or more target biological molecule(s) from a biological composition by dynamic packed bed adsorption using a separation medium comprising porous rod-shaped particles linking to the target molecule, and filtration using filter having a pore size of at least 1.5 μm.
Claims16
A method for isolating one or more target biological molecule(s) from a biological composition comprising the steps of
- a) providing a separation medium in the form of porous rod-shaped particles that have been derivatised with functional ligands to interact with target biological molecule(s) in a mobile liquid phase, and wherein the porous rod-shaped particles have an average length from 50 μm to 10,000 μm and average diameter from 1 μm to 350 μm;
- b) mixing the separation medium with a biological composition comprising one or more target biological molecule(s), and whereby the one or more target biological molecule is adsorbed onto the separation medium;
- c) filtrating the mixture of separation medium and biological composition using a filter to form a dynamic packed bed of the separation medium on the filter, wherein the nominal pore size of the filter is at least 1.5 μm, and wherein the filter is a tangential flow filter or the filter is a flat sheet or flat screen being vibrated or shaked during filtration;
- d) optionally, eluting the target biological molecule from the separation medium while the separation medium is being retained by said filter.
An apparatus for isolating one or more target biological molecule(s) from a biological composition, the apparatus comprising:
- a first inlet 1 to a mixing tank 2 comprising a separation medium in the form of porous rod-shaped particles that have been derivatised with functional ligands to interact with target biological molecule(s) in a mobile liquid phase, and wherein the porous rod-shaped particles have an average length from 50 μm to 10,000 μm and average diameter from 1 μm to 350 μm;
- said mixing tank 2 having one or more first fluid connections 3 leading fluid from the first inlet 1 to a first end 4 of a filtration unit 5 comprising a filter 6 with a pore size being at least 1.5 μm and wherein the filter is a tangential flow filter or the filter is a flat sheet or flat screen being vibrated or shaked during filtration;
- and wherein a second end 7 of the filtration unit 5 has a second fluid connection 8 leading retentate back to the mixing tank 2 and the first end of the filtration unit 5;
- the filtration unit 5 having a third fluid connection 9 leading permeate to a first outlet 10.
Dependencies are inferred from the claim text.