Epithelial cell tube for tracheal transplantation
AssigneeSeoul National University R&Db Foundation
InventorsHYUN WOO SHIN, KHALMURATOVA ROZA
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Abstract
In a method for preparation of an epithelial cell tube, epithelial cells are cultured at an air-liquid interface cell culture system to obtain differentiated epithelial cells, the differentiated epithelial cells as a layer are transferred and adhered to a planar support so that cilia of the epithelial cells face a lumen side, and both ends of the planar support are linked to each other so that the layer of epithelial cells faces an outer peripheral surface of the planar support, resulting in forming the epithelial cell tube comprising a columnar support and the layer of differentiated epithelial cells adhered on the columnar support.
Claims8
A method for preparation of an epithelial cell tube, the method comprising:
- (i) culturing epithelial cells at an air-liquid interface cell culture system to obtain differentiated epithelial cells;
- (ii) transferring and adhering the differentiated epithelial cells as a layer to a planar support so that cilia of the epithelial cells face a lumen side;
- and
- (iii) linking both ends of the planar support each other so that the layer of epithelial cells faces an outer peripheral surface of the planar support, resulting in forming the epithelial cell tube comprising a columnar support and the layer of differentiated epithelial cells adhered on the columnar support.
Dependencies are inferred from the claim text.