Bacteriophages as a tool to manipulate gut commensal immune-modulation activity
AssigneeTechnion Research & Development Foundation Limited
InventorsNaama GEVA-ZATORSKY, Tal GEFEN, Dana KARITI, Nadav BEN-ASSA, Robert ABBOUD, Shaqed CARASSO, Rawan ZAATRY
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Abstract
The present disclosure relates to the microbiome field. More specifically, the present disclosure relates to bacteriophages as a tool to manipulate gut immune-modulatory activity and uses thereof for therapy. More specifically, the present disclosure provides methods and tools for modulating an immune response in a subject in need thereof, by applying bacteriophage that modulates the phenotype of at least one bacteria in a subject.
Claims21
-50. (canceled)
A method for modulating an immune response in a subject in need thereof, the method comprising the step of administrating to said subject at least one bacteriophage that modulates the phenotype of at least one bacteria in said subject, wherein at least one of:
- (i) the bacteriophage modulates phase variation of at least one locus of said at least one bacteria;
- and/or
- (ii) said bacteriophage comprises at least one recognition sequence for at least one bacterial restriction modification system (R-M), that is specifically recognized by at least one target recognition component of said R-M system of at least one bacterium displaying a desired phenotype;
- and/or
- (iii) the bacteriophage modulates bacterial functionality.
An engineered bacteriophage comprising at least one exogeneous recognition sequence of at least one bacterial restriction modification system (R-M), or a composition at least one of said engineered bacteriophage or any cocktail or mixture of said bacteriophages or any vehicle, matrix, nano- or micro-particle thereof;
- wherein said at least one recognition sequence is specifically recognized by at least one component of said restriction modification system of at least one bacterium displaying a desired phenotype, said recognition sequence comprises a 5′ recognition site and a 3′ recognition site separated by 3 to 10 unspecified intervening nucleotides, and wherein said composition further comprises at least one of pharmaceutically acceptable carrier/s, diluent/s, excipient/s and additive/s.
A method for modulating the phenotype of a bacterial population, the method comprising the step of contacting said bacterial population with an effective amount of at least one engineered bacteriophage comprising at least one exogeneous recognition sequence of at least one bacterial restriction modification system (R-M), wherein said at least one recognition sequence is specifically recognized by at least one component of said restriction modification system of at least one bacterium displaying a desired phenotype, thereby enriching bacteria displaying said desired phenotype in the bacterial population, wherein said recognition sequence comprises a 5′ recognition site and a 3′ recognition site separated by 3 to 10 unspecified intervening nucleotides.
Dependencies are inferred from the claim text.