Biocatalytic synthesis of unnatural mycosporine-like amino acids
AssigneeUniversity of Florida Research Foundation, Inc.
InventorsYousong Ding, Manyun Chen
Life of this patent
Estimating the term…
Abstract
The present invention relates to methods of producing compounds of interest in a recombinant microorganism. In particular, the present invention relates to using a recombinant microorganism comprising a heterologous nucleic acid encoding one or more mycosporine-like amino acid (MAA) biosynthetic enzymes (e.g., MysD) to produce compounds of interest using unnatural amino acids or amine containing substrates. Compositions comprising compounds produced using such methods are also provided herein. The present disclosure also provides methods of preventing sunburn, cancer, and chronic inflammatory diseases by administering such compositions to subjects in need thereof.
Claims73
A method for producing a compound, comprising:
- a) culturing a recombinant microorganism under conditions suitable for production of the compound;
- and
- b) isolating the compound from the recombinant microorganism,
- wherein the recombinant microorganism comprises a heterologous nucleic acid encoding one or more mycosporine-like amino acid (MAA) biosynthetic enzymes, wherein the one or more MAA biosynthetic enzymes comprise a D-alanine-D-alanine ligase (MysD), or a homolog thereof.
A recombinant microorganism comprising a heterologous nucleic acid encoding one or more mycosporine-like amino acid (MAA) biosynthetic enzymes, wherein the one or more MAA biosynthetic enzymes comprise a D-alanine-D-alanine ligase (MysD), or a homolog thereof.
A compound produced by:
- a) culturing a recombinant microorganism under conditions suitable for production of the compound;
- and
- b) isolating the compound from the recombinant microorganism,
- wherein the recombinant microorganism comprises a heterologous nucleic acid encoding one or more mycosporine-like amino acid (MAA) biosynthetic enzymes, wherein the one or more MAA biosynthetic enzymes comprise a D-alanine-D-alanine ligase (MysD), or a homolog thereof.
Dependencies are inferred from the claim text.