Method of improving stability of dentin bonding interface using phosphorylated chitosan

Abstract

The present disclosure provides a method of improving stability of a dentin bonding interface using phosphorylated chitosan, belonging to the technical field of biomedicine. The method according to the present disclosure includes: applying a mixture of phosphorylated chitosan, 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide (EDC), and N-hydroxysuccinimide (NHS) uniformly onto a dentin surface to form a covalently cross-linked network and induce in situ deposition of hydroxyapatite. The present disclosure achieves the following performance enhancements to the dentin bonding interface: the present disclosure increases the mechanical strength and enzymatic degradation resistance of dentin collagen fibers. The present disclosure imparts bacterial inhibition capability to the bonding interface, thereby preserving demineralized dentin collagen fibers and stabilizing the bonding interface. The present disclosure stabilizes the dentin collagen fibers, maintains a microenvironment suitable for remineralization, promotes the deposition of minerals such as calcium and phosphorus on the dentin surface, and also provides protection for the dentin against demineralization.

Dependencies are inferred from the claim text.