Customized wearable saliva biosensor for real-time continuous health monitoring

A method for continuous real-time monitoring of salivary biomarkers comprising:

• positioning a wearable saliva biosensor system within the oral cavity of a user such that an OECT biosensor of the system is located in proximity to the sublingual glands and contacts fresh saliva at the point of secretion;
• simultaneously applying a gate voltage to an enzyme-functionalized gate electrode and a control gate electrode of the OECT biosensor;
• measuring a differential electrical signal between the enzyme-functionalized gate electrode and the control gate electrode representing the concentration of a target biomarker in the saliva to create a data stream;
• wirelessly transmitting the data stream to an external device;
• and
• converting the data stream into a biomarker concentration value.

A method of fabricating a customized wearable saliva biosensor system comprising:

• obtaining a three-dimensional scan of a dental model of a user's oral anatomy;
• generating a digital substrate model from the three-dimensional scan customized to the user's oral anatomy;
• fabricating a wearable substrate from the digital substrate model using a biocompatible material;
• integrating an OECT biosensor, a wireless integrated circuit, and a power source into the wearable substrate;
• and
• encapsulating the OECT biosensor, wireless integrated circuit, and power source with a biocompatible encapsulant to form a unitary sensing platform within the wearable substrate.