US 2026/0271926 A1 · BACTERIA-RESPONSIVE PARTICLES, METHODS FOR PREPARATION, AND USE IN PACKAGING OR ON SURFACES

Abstract

Bacteria-responsive particles and a platform for product packaging or for incorporating on surfaces are described, together with uses thereof and methods of preparation. The particles, such as liposomes or lipid nanoparticles, provide a bacteria-responsive platform for release of an agent, which may be antimicrobial, only in the presence of the target bacteria upon release of virulence factors referred to as cholesterol-dependent cytolysins (CDCs) by the bacteria. Common bacteria involved in food poisoning, such as bacillus, Clostridium, listeria, and streptococcus, produce CDCs and can be targeted with the particles described. The particles are cholesterol-rich, and comprise surface-exposed cholesterol. The releasable agent is encapsulated within the particles. Interaction of the surface-exposed cholesterol with the CDC released from the target bacteria causes particle porosity and release of the agent encapsulated within the particles, permitting the agent to take effect, such as to deter the target bacteria or indicate its presence.

Claims30

Dependent claims

Bacteria-responsive particles encapsulating a releasable agent for interaction with a target bacteria that releases a cholesterol-dependent cytolysin (CDC), said particles comprising:

surface-exposed cholesterol, and
said releasable agent encapsulated within the particles, for interaction with the target bacteria upon release,
wherein interaction of the surface-exposed cholesterol of the particles with the CDC released from the target bacteria causes particle porosity and release of the agent from the particles.

A method of preparing bacteria-responsive particles encapsulating a releasable agent for interaction with a target bacteria, when the target bacteria releases a cholesterol-dependent cytolysin (CDC), the method comprising:

preparing a particle-forming composition for providing particles with a lipid-rich total cholesterol content of from 30-85 mol %, from 55-75 mol %, or from 60-70 mol %, said composition comprising the releasable agent;
sonicating the composition for 80 s to 600 s, such as about 200 s;
forming particles having an average diameter of from 50 to 1000 nm, an average diameter of from 100 nm to 500 nm, an average diameter of from 300 to 400 nm, or having an average diameter of about 300 nm;
filtering the particles to exclude sizes over about 35 kDa, over about 30 kDa, or over about 20 kDa;
and
washing the particles to remove non-encapsulated agent;
wherein the prepared particles comprise surface-exposed cholesterol, and the releasable agent is encapsuled within said particles.

4 more elements

Dependencies are inferred from the claim text.

Bacteria-responsive particles, methods for preparation, and use in packaging or on surfaces

Life of this patent

Bacteria-responsive particles, methods for preparation, and use in packaging or on surfaces

Life of this patent

Abstract

Claims30